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Tgf β1 elisa kit

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The TGF-β1 ELISA kit is a laboratory equipment product designed to quantitatively measure the concentration of transforming growth factor beta 1 (TGF-β1) in biological samples. It is a sensitive and reliable tool for researchers to assess the levels of this important cytokine in various applications.

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Lab products found in correlation

Complete mini, by Roche (1 mentions) Tissue extraction reagent 1, by Thermo Fisher Scientific (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Precellys 24 homogenizer, by Bertin Technologies (1 mentions) Cholesterol quantitation kit, by Merck Group (1 mentions) Mcpt 1 mmcp 1 elisa kit, by Thermo Fisher Scientific (1 mentions) Il 1beta elisa kit, by Thermo Fisher Scientific (1 mentions) Trichrome stain masson kit, by Merck Group (1 mentions) Anti mouse ly6c, by BioLegend (1 mentions) Anti mouse ly6g, by BioLegend (1 mentions) Anti mouse tnf α, by BioLegend (1 mentions) Bio plex software, by Bio-Rad (1 mentions) Dc protein assay kit, by Bio-Rad (1 mentions) Vegf elisa development kit, by Thermo Fisher Scientific (1 mentions) Il 6 duoset elisa, by R&D Systems (1 mentions) Bulletkits, by Lonza (1 mentions) Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions) Du145, by American Type Culture Collection (1 mentions) 3 3 diaminobenzidine dab peroxidase substrate kit, by Vector Laboratories (1 mentions) 22rv1 cell, by American Type Culture Collection (1 mentions)

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TGF-β1 (1092 citations) ELISAs (35 citations)

19 protocols using tgf β1 elisa kit

1

Quantifying TGF-β1 in Normoxic and Hypoxic Cells

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Human TGF-β1 was measured in normoxic and hypoxic cells or MVs using the TGF-β1 ELISA kit (eBioscience).
Berchem G., Noman M.Z., Bosseler M., Paggetti J., Baconnais S., Le cam E., Nanbakhsh A., Moussay E., Mami-Chouaib F., Janji B, & Chouaib S. (2015). Hypoxic tumor-derived microvesicles negatively regulate NK cell function by a mechanism involving TGF-β and miR23a transfer. Oncoimmunology, 5(4), e1062968.
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2

Quantifying TGF-β1 in Frozen Tumors

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Frozen tumors were cut into small pieces and homogenized using Precellys 24 homogenizer (Bertin Technologies, Rockville, MD, USA) in Tissue Extraction Reagent I (Invitrogen) containing protease inhibitor cocktail (Complete Mini; Roche, Basel, Switzerland). Supernatant of the homogenate was obtained by centrifugation and stored at −70°C until analysis. Total and active TGF‐β1 concentrations were determined using TGF‐β1 ELISA kit (eBioscience, Vienna, Austria) according to the manufacturer's protocol and total protein concentration using BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA).
Kim J.H., Shin B.C., Park W.S., Lee J, & Kuh H. (2017). Antifibrotic effects of pentoxifylline improve the efficacy of gemcitabine in human pancreatic tumor xenografts. Cancer Science, 108(12), 2470-2477.
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3

Hepatic Fibrosis Quantification Protocol

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The HFD was purchased from Harlan Laboratories, Inc (Dublin, VA). Oil‐Red‐O staining kit was from Newcomer Supply (Middleton, WI). Trichrome Stain (Masson) Kit and Cholesterol Quantitation Kit were from Sigma‐Aldrich (Louis, MO). Triglyceride Quantification Colorimetric/Fluorometric Kit and Alanine Aminotransferase (ALT or SGPT) Activity Colorimetric/Fluorometric Assay Kit were from BioVision Incorporated (Milpitas, CA). Mouse LIPA/Lysosomal Acid Lipase Sandwich ELISA Kit was purchased from LSBio Lifespan BioSciences, Inc (Seattle, WA). Mouse tumor necrosis factor‐α (TNF‐α) ELISA Kit, interleukin (IL)–6 ELISA, IL‐1 beta ELISA Kit, MCPT‐1 (mMCP‐1) ELISA Kit, and human/mouse transforming growth factor‐β (TGF‐β) 1 ELISA Kit were purchased from eBioscience (San Diego, CA). Anti‐mouse alpha smooth muscle actin (MSC), anti‐mouse F4/80 and anti‐SQSTM1/62 antibodies were from Abcam (Cambridge, MA). The anti‐mouse Ly6G, anti‐mouse Ly6C, anti‐mouse CD11b, anti‐mouse C‐X‐C motif chemokine receptor 2, and anti‐mouse TNF‐α were from BioLegend (San Diego, CA). Anti‐mouse IL12p40 and anti‐mouse Gr‐1 antibody was from eBioscience (San Diego, CA).
Chen K., Yuan R., Zhang Y., Geng S, & Li L. (2017). Tollip Deficiency Alters Atherosclerosis and Steatosis by Disrupting Lipophagy. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 6(4), e004078.
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4

Cytokine Profiling of Hypoxic CAFs

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Human TGF-β concentration was measured in normoxic and hypoxic CAF CMs using a TGF-β1 ELISA kit (eBiosciences). Human VEGF, IL6, IL10, CCL2, CCL5 and IFN-γ concentrations were simultaneously measured in normoxic or hypoxic CAF CMs using a Bio-Plex ProTM human cytokine Multiplex Immunoassay, according to the manufacturer protocol. Data were analyzed using a Bio-Plex 200 instrument and Bio-Plex software (Biorad).
Ziani L., Buart S., Chouaib S, & Thiery J. (2021). Hypoxia increases melanoma-associated fibroblasts immunosuppressive potential and inhibitory effect on T cell-mediated cytotoxicity. Oncoimmunology, 10(1), 1950953.
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5

Bleomycin-Induced Lung Injury: Quantification of LRG and TGF-β1

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Mice were sacrificed on days 1, 7, 14, and 21 after bleomycin treatment, and the trachea was cannulated with a 20‐gage indwelling needle. Lungs were lavaged three times with 0.5 mL of PBS for each lavage. The lavage fluids were centrifuged at 400g for 5 min at 4°C, and the supernatants were stored at −80°C until the measurement of LRG and TGF‐β1 concentrations. Total cells in BALF were counted using a hemocytometer. Protein concentration in the supernatant of BALF was measured using a DC Protein Assay Kit (Bio‐Rad Laboratories, Hercules, CA, USA). Concentrations of LRG and TGF‐β1 were measured by ELISA. For the measurement of LRG, monoclonal antibodies mLRA0010 and rLRA0094 were used as previously described (Honda et al. 2016). The specificity of these antibodies was confirmed using the BALF and serum from a LRG KO mouse. The concentrations of BALF TGF‐β1 were measured according to the manufacturer's protocols using the TGF‐β1 ELISA kit (eBioscience, San Diego, CA).
Honda H., Fujimoto M., Serada S., Urushima H., Mishima T., Lee H., Ohkawara T., Kohno N., Hattori N., Yokoyama A, & Naka T. (2017). Leucine‐rich α‐2 glycoprotein promotes lung fibrosis by modulating TGF‐β signaling in fibroblasts. Physiological Reports, 5(24), e13556.
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6

Quantifying TGF-β1 Secretion

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Cells were grown in complete medium and treated with or without JNK inhibitor (30 μM) for 16 h and then incubated for 24 h in serum‐free medium. Cell culture supernatants were assayed using a TGF‐β1 ELISA kit (eBioscience, San Diego, CA, USA) according to the manufacturer's instructions. The absorbance was measured at a wavelength of 450 nm.
Wang J., Ni W., Hu K., Zhai X., Xie F., Jie J., Zhang N., Jiang L., Yuan H, & Tai G. (2017). Targeting MUC1 and JNK by RNA interference and inhibitor inhibit the development of hepatocellular carcinoma. Cancer Science, 108(3), 504-511.
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7

Quantification of Kidney TGF-β1

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Kidney cortices were dissected and homogenized in denaturing lysis buffer containing 100 mM NaCl, 1% Triton X-100, and 0.05 M Tris-HCl, pH7.4. Protein concentrations were measured using a BCA method according to the manufacturer's instruction. Total TGF-β1 was quantified using a TGF-β1 ELISA kit (Invitrogen). Values were expressed as pg/mg protein for the protein extracts.
Sun Y., Zhou G., Gui T., Shimokado A., Nakanishi M., Oikawa K., Sato F, & Muragaki Y. (2014). Elevated serum 1,25(OH)2-vitamin D3 level attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction in kl/kl mice. Scientific Reports, 4, 6563.
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8

Bioactive Factors Secretion Analysis

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After 5 days in culture, DP cell medium was replaced by the equivalent FBS-free media and cultured for 24 h. The supernatant was then collected, centrifuged (3000 rpm, 10 min) and stored at −80 °C in single-use aliquots. VEGF ELISA Development Kit, BMP2 Standard ELISA Development Kit (Peprotech), PDGF-AA DuoSet ELISA, IL-6 DuoSet ELISA (R&D Systems) and TGF-β1 Elisa kit (Invitrogen) were used according to the manufacturer’s instructions. Control wells with FBS-free media incubated in the same conditions but without cells were used to determine the amount of each biomolecule in the different media used.
Abreu C.M., Cerqueira M.T., Pirraco R.P., Gasperini L., Reis R.L, & Marques A.P. (2020). Rescuing key native traits in cultured dermal papilla cells for human hair regeneration. Journal of Advanced Research, 30, 103-112.
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9

Quantifying TGF-β1 Secretion in PC-9 Cells

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The 24‐hour cell‐free supernatant of tissue culture from PC‐9 with or without APE1 overexpression and PC‐9/ER with or without APE1 knockdown were collected 48 hours post‐transfection or infection and measured using a TGF‐β1 ELISA kit (Invitrogen) following the manufacturer's instruction. The absorbance of each well was read at 450 nm.
Yang X., Peng Y., Jiang X., Lu X., Duan W., Zhang S., Dai N., Shan J., Feng Y., Li X., Cheng Y., Yang Y., Baugh L., Tell G., Wang D, & Li M. (2018). The regulatory role of APE1 in epithelial‐to‐mesenchymal transition and in determining EGFR‐TKI responsiveness in non‐small‐cell lung cancer. Cancer Medicine, 7(9), 4406-4419.
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10

Prostate Cancer Cell Line Characterization

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Human PCA PC3, DU-145, LNCaP and 22Rv1 cells were from ATCC. C4-2B cells were from ViroMed Laboratories. PCA cell lines were tested and authenticated by DNA profiling for polymorphic short tandem repeat (STR) markers at University of Colorado Cancer Center DNA Sequencing & Analysis Core. RPMI 1640 media, other cell culture materials, TGFβ1 ELISA kit, and CAS-Block were from Invitrogen. PrSCs, SCGM, and Bullet-kits were from Lonza. Prostate CAFs were from a prostatectomy specimen removed at Wake Forest University [13 (link)]. Pathology of specimen was verified by two board-certified pathologists (AC and JS). No patient identifiers were retained and use of discarded tissue was not considered human subjects research by Wake Forest University IRB. DAPI and silibinin were from Sigma, IL-6 from Cell Signaling, and TGFβ1, TGFβ2, and goat IgG isotype control antibody were obtained from Gibco. TGFβ2 ELISA kit and TGFβ2-neutralizing antibody were obtained from R&D, antibodies to IL-6, α-SMA and FAP (fibroblast activation protein) from Abcam, antibody to vimentin and HRP conjugated streptavidin from Santa Cruz, 3,3′-diaminobenzidine (DAB) peroxidase substrate kit from Vector Labs, biotinylated antibodies and mouse IgG's from DAKO, and transwell invasion chambers from BD Biosciences.
Ting H., Deep G., Jain A.K., Cimic A., Sirintrapun J., Romero L.M., Cramer S.D., Agarwal C, & Agarwal R. (2014). Silibinin Prevents Prostate Cancer Cell-Mediated Differentiation of Naïve Fibroblasts into Cancer-Associated Fibroblast Phenotype by Targeting TGF β2. Molecular carcinogenesis, 54(9), 730-741.
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