RAW 264.7 cell migration assays were performed in transwell migration assay chambers (BD Life Sciences, Franklin Lakes, NJ, USA) by adding adipocyte-derived CM to the lower chamber. Raw 264.7 cells incubated with vehicle, RhoA or RhoB for 3 h were seeded onto transwell and the migrated cells towards adipocyte CM were counted after 3 h exposure.
Transwell migration assay chambers
Manufactured by BD
Sourced in United States
Transwell migration assay chambers are a cell culture insert system used to study cell migration and invasion. The chambers consist of an upper and lower compartment separated by a porous membrane that allows cells to migrate from the upper to the lower chamber.
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Lab products found in correlation
3 protocols using transwell migration assay chambers
1
Adipocyte-Induced Cell Migration Assay
2
Macrophage Migration Assay with FLS
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Cell migration and invasion assays were performed using transwell migration assay chambers (BD Life Sciences, Franklin Lakes, NJ, USA) with an 8-μm pore size. Bone marrow macrophages isolated from WT or mS6KO mice were layered onto a transwell insert, and migration was assessed with fibroblast-like synoviocyte (FLS) conditioned medium (CM), MCP-1, or RANTES present in the lower well.
3
Plasmid Transfection and Cell Migration Assay
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To express exogenous proteins, PC-3 cells were mock-transfected or transfected with 0.5 to 4 μg LDHA-WT or LDHA-mutant using lipofectamine 3000 (Catalog No. L3000-008, Invitrogen, Carlsbad, CA). For the migration assay, cell migration was analyzed using transwell migration assay chambers (BD Life Sciences). PC-3 cells were transfected with 4 μg plasmid DNA and incubated for 24 h. Then, 1.5 × 105 cells were transferred to transwell chambers to perform a migration assay and incubated for 24 h on transwell chambers.
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