An Olympus IX73 microscope and MicroPoint pulsed laser system (Andor Technology) are used for these experiments. The laser is set to a power of 20 with 99 pulses for venous ablation and a power of 19 with 30 pulses for arterial ablation. This system utilizes a nitrogen laser pumped through coumarin dye, which is attached to an Olympus IX73 inverted microscope fitted with a GFP filter for visualizing fluorescently labeled thrombocyte aggregation. For venous laser injury, embryos are observed using a 20× objective, and a 40× objective is used for arterial laser injury.
Ix73 microscope
Manufactured by Oxford Instruments
The IX73 microscope is a high-performance inverted microscope designed for various applications in life science research. It features a stable and ergonomic design, providing a comfortable user experience. The IX73 offers a range of optical capabilities, including phase contrast, differential interference contrast (DIC), and fluorescence imaging, allowing researchers to visualize and analyze a wide variety of samples.
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Lab products found in correlation
3 protocols using ix73 microscope
1
Laser-Induced Thrombocyte Aggregation
2
Live Bacterial Cell Conjugation Imaging
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Bacterial cells were grown as indicated previously. Samples were placed onto a cover glass–bottom imaging dish, with the bacteria sandwiched between the agarose pads and the cover glass. The dish was placed into a custom stage insert, which holds the dish tightly. After sealing with ParafilmR or grease, bacteria cells were seeded onto individual agarose pads and incubated for 10 min at 37°C.
Live conjugation was monitored in a Nikon Eclipse Ti2 microscope equipped with a Hamamatsu ORCA-Flash 4.0 camera using a CFI Apochromat TIRF100xc Oil objective. For TIRF imaging, a Nikon A1 confocal laser microscope equipped with Hamamatsu 9100-C2 camera and a Plan Apochromat TIRF 100x Oil DIC HN2 objective was used. Super-resolution imaging (d-STORM) was performed in an Olympus IX-73 microscope equipped with 100xoTIRF Olympus UAPON objective and an iXon ULTRA897 EMCCD camera (Andor). Image analysis was carried out using Fiji/ImageJ software (National Institutes of Health, United States).
Live conjugation was monitored in a Nikon Eclipse Ti2 microscope equipped with a Hamamatsu ORCA-Flash 4.0 camera using a CFI Apochromat TIRF100xc Oil objective. For TIRF imaging, a Nikon A1 confocal laser microscope equipped with Hamamatsu 9100-C2 camera and a Plan Apochromat TIRF 100x Oil DIC HN2 objective was used. Super-resolution imaging (d-STORM) was performed in an Olympus IX-73 microscope equipped with 100xoTIRF Olympus UAPON objective and an iXon ULTRA897 EMCCD camera (Andor). Image analysis was carried out using Fiji/ImageJ software (National Institutes of Health, United States).
3
Steady-state Localization of Tau Proteins
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The steady-state localization of tau and other marker proteins was documented using an Olympus IX73 microscope with a 60×/1.42 NA objective lens and a Andor Zyla5.5 camera, or a Carl Zeiss ApoTome system with a 63×/1.4 NA lens. A line scan analysis of fluorescence intensity was performed using ImageJ.
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