All vaginal swabs (ESwab ® transport medium, Copan Italia, Brescia, Italy) were plated onto Columbia colistin-nalidixic acid agar (bioMérieux, Marcy l'Etoile, France) and incubated overnight at 35°C. Identification was performed by means of Vitek ® MS MALDI-TOF system (bioMerieux, Marcy l'Etoile, France). Briefly, an amount of the microbial growth from the agar plate was spotted onto the MALDI-TOF target plate, immediately overlaid with 1 μl of matrix solution and air dried. The target plate was then placed into the MALDI-TOF MS device and underwent identification. The Vitek ® MS system identifies the organism by means of an advanced spectrum classifier algorithm that calculates a confidence value as a percent probability that represents the similarity in terms of presence/absence of specific peaks between the generated spectrum and the database spectra (Dubois et al, 2012) .
Eswab transport medium
Manufactured by Copan
Sourced in Italy
ESwab ® is a transport medium designed for the collection, transport, and preservation of clinical specimens. It consists of a nylon-flocked swab and a tube containing a liquid Amies medium. The medium helps maintain the viability of aerobic and anaerobic bacteria, as well as some viruses, during transport to the laboratory for analysis.
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Lab products found in correlation
3 protocols using eswab transport medium
1
Rapid Bacterial Identification via MALDI-TOF
2
Multiplex PCR Analysis of Respiratory Viruses
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Included in this report were respiratory specimens collected by 15 October 2013 that were available for testing by high-throughput, quantitative, multiplex PCR (HT-qPCR) assay developed for 13 respiratory viruses [13 (link)]. NP swab specimens were collected by trained personnel during the monthly visit by introducing a flocked swab (FLOQSwabs, COPAN, Murrieta, California) into the nose until resistance was met. The swab was rotated gently 180° and then placed in a 1-mL tube of ESwab transport medium (COPAN Diagnostics, Murrieta, California) and transported to the lab on ice within 1 hour of collection. An aliquot was sent for bacterial culture; the remaining aliquots were kept frozen at −80°C until testing. During a URTI episode, additional nasopharyngeal secretion (NPS) samples were also collected using vacuum suction into a mucus trap as previously described [13 (link)].
3
Microbial Sampling and Analysis
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Samples for microbiological investigations were collected every Monday and Wednesday after 5 pm with flocked swabs moistened with NaCl solution prior to use and after swabbing put in eSwab® transport medium (Copan, Brescia, Italy). Guided by clean 25.2 cm2 metal templates, the test foil as well as the control surfaces were swabbed. The swabs were immediately brought to the microbiology laboratory and stored at 4–8 °C overnight before processing.
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