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7 protocols using β ocimene

1

Synthetic Standards for EAD-Active Compounds

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The synthetic standards of the following EAD-active compounds were used: hexanal (Sigma Aldrich, 99%), (E)-2-hexenol (Aldrich, 96%), benzaldehyde (Sigma Aldrich, 99.5), β-myrcene (Sigma Aldrich, 99%), ocimene (International Flavors and Fragrance, New York, USA, (Z)-β-ocimene = 27%, (E)-β-ocimene = 67% and allo-ocimene = 6%), linalool oxide (Sigma Aldrich, mixture of stereoisomers with furanoid form, 99.5% and 0.5% pyranoid form), indole (Sigma Aldrich, 99%) and 1-octen-3-ol (Fluka Chemica, racemic mixture of R and S 98%).
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2

Investigating β-ocimene Interactions

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Ten microliters of 0.1 M β-ocimene (Sigma-Aldrich Co.) was applied onto a cotton swab, which was then hung in a glass jar (3-L) containing a Chinese cabbage plant. Given that the β-ocimene, we used dissolved in CH2Cl2, we used CH2Cl2 treated plants as controls. Plant treated with water only were used as negative controls. Twenty-four hours later, these treated plants were used for the investigation.
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3

Comparative Analysis of Tillandsia xiphioides Ecotypes

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All the flowers of T. xiphioides studied in this report come from the
Tillandsia PROD plant nursery located in the commune of Le Cailar
(Occitania, France). The plant nursery includes about 500,000 Tillandsia,
of 350 species, varieties, forms, and hybrids. T. xiphioides is known to present many ecotypes differing on pubescence from lepidote
mentose with dense wolly hairs, among other characteristics.23 For the PCA, two forms of T.
xiphioides
called in this paper Low-Pubescent T. xiphioides (LP-xiphi) and High-Pubescent T. xiphioides (HP-xiphi) according to, respectively,
weak and strong morphological development of trichomes on the leaves,
were used (see Figures S1 and S2 in Supporting Information). A total of 10 plants of each form providing 2–3
flowers each were harvested from February to May 2020.
All chemicals
were purchased from Sigma-Aldrich (St. Louis, USA): α-pinene
(98.5% purity), sabinene (75%), β-pinene (98.5%), β-myrcene
(90%), limonene (99%), eucalyptol (99%), β-ocimene (95.4%),
γ-terpinene (98.5%), β-linalool (97%), α-terpineol
(90%), geraniol (99%), geranyl acetate (99%), and nerolidol (98.5%).
All have an analytical standard grade except for sabinene.
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4

Quantitative Analysis of Volatile Compounds

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The following reference standards were purchased from Sigma-Aldrich (St. Louis, MO, USA): C7-C30 saturated alkane mixed standard, (internal standard) 2-methyl-3-heptanone, 2-methylpropyl 2-methylpropanoate, β-myrcene, β-trans-ocimene, ethyl hexanoate, 3-octanone, hexyl acetate, 2-methylbutyl 2-methylbutanoate, (Z)-3-hexenyl acetate, (Z)-hex-3-en-1-ol, 2-nonanone, 3-octanol, ethyl octanoate, (Z)-3-hexenyl butanoate, α-gurgujene, linalool, β-caryophyllene, (Z)-3-hexenyl (E)-2-butenoate, 2-undecanone, methyl benzoate, (Z)-3-hexenyl hexanoate, ethyl benzoate, humulene, α-farnesene, (Z)-3-hexenyl benzoate, and β-ocimene. All chemicals were of analytical grade or higher.
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5

Comprehensive Fragrance Compound Analysis

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Humulene, 2-penten-1-ol, 2-hexen-1-ol, (E)-3-hexen-1-ol, 1-hexanol, p-cymen-8-ol, 2-vinyloxy)-ethanol, 3-methyl-1-butanol, 1,3,8-p-menthatriene, allo-ocimene, 2-carene, α-phellandrene, 3-carene, terpinolene, 1,3-cyclohexadiene, 1-methyl-4-(1-methylethyl), isovaleraldehyde, 3-hexenal, 2,4-dimethyl-benzaldehyde, heptanal, trans-2-heptenal, trans-2-pentenal, 1-nonanal, decanal, citral, 1-penten-3-one, 2-cyclohepten-1-one, 3-methylcyclohex-3-en-1-one, 6-methyl-5-hepten-2-one, ethyl-propionate, ethyl cyclopropanecarboxylate, ethyl crotonate, isoamyl acetate, tetraethyl orthosilicate, ethyl butyrate, and γ-octanoic were purchased from TCI (Tokyo, Japan); and linalool, α-pinene, β-pinene, β-myrcene, D-limonene, p-cymene, β-ocimene, hexanal, γ-terpinene, and trans-2-hexenal were purchased from Sigma (St. Louis, MO, USA). All of the chemical standards were of GC quality.
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6

Comprehensive Terpenoid Analysis Protocol

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Limonene (≥95%), β-ocimene (95%), (E)-2-heptenal (95%), 1,8-cineole (99%), carvone (≥95%), β-myrcene (95%), linalool (98%), linalool oxide (97%), and n-alkane (C6-C30, ≥99%) standards and the internal standard 4-octanol (≥99%) were purchased from Sigma-Aldrich (Shanghai, China). Dichloromethane and sodium sulfate were purchased from Thermo Fisher (Beijing, China). Enantiomer standards including S-(-)-Limonene (96%), R-(+)-Limonene (97%), R-(-)-linalool (97%), R-(-)-carvone (97%), and S-(+)-carvone (97%) were purchased from Sigma Aldrich (Shanghai, China).
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7

Analysis of Volatile Compounds in Tea

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Five-year-old tea plants of Camellia sinensis cv. “Shu Cha Zao” grown at the experimental farm of Anhui Agricultural University in Hefei, China, were used in this study. Standards of geranyl diphosphate (GDP), farnesyl diphosphate (FDP), β-myrcene, β-ocimene, linalool, geraniol, α-citral, β-citral, methyl geranate, nerol, D-limonene, trans-linalool oxide (furanoid), neryl acetate, L-terpineol, α-terpinen, geranyl acetone, thymol, (Z)-nerolidol, α-farnesene, β-farnesene, (E)-nerolidol, humulene, α-ionone, β-ionone, (E)-γ-bisabolene, cadinol and caryophyllene were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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