Finnigan surveyor plus system

A Finnigan Surveyor Plus system (Thermo Fisher Scientific, Waltham MA, USA) consisting of a Finnigan Surveyor Autosampler Plus, an MS Pump Plus, a PDA Plus detector, a Thermo LTQ ESI MS system (Thermo Fisher Scientific), and a CD-2095 Plus Chiral Detector (JASCO) was used.

LC–MS analysis was performed using a Finnigan Surveyor plus system (Thermo Scientific, San Jose, CA, USA), equipped with a quaternary MS pump with an integrated degasser and an autosampler with an integrated column oven. The chromatographic separation was performed on a Kinetex C18 column (5 µm, 50 mm × 4.6 mm ID) preceded by a C18 security guard cartridge (4.0 mm × 3.0 mm ID), both from Phenomenex (Torrance, CA, USA). Separation was attained using an isocratic elution with the flow rate of 0.5 ml/min. The column and tray temperature was set at 25°C and 10°C, respectively. The mobile phase consisted of 0.5% formic acid in water and methanol (45:55 v/v). The run time of the analysis was 3 min.
Mass spectrometry was performed on an LTQ XL linear ion trap instrument (Thermo Scientific, San Jose, CA, USA), coupled with heated electrospray ionization (HESI-II) probe operated in the positive ion mode. After optimization, the parameters in the source were set as follows: source voltage of 4.5 kV, source heater temperature of 250°C, sheath gas flow of 30 arb, and auxiliary gas flow of 10 arb. The mass spectrometer was operated in the selected reaction monitoring (SRM) channels. Fragment ion m/z 180 → 163 was used for memantine quantification, while m/z 152 → 135 was used for amantadine quantification. Thermo Fisher Xcalibur software was used for the analysis.

LC-MS analysis was carried out using a Finnigan Surveyor plus system (Thermo Scientific, San Jose, CA, USA), consisting of a quaternary MS pump with integrated degasser and an auto sampler with integrated column oven. The chromatographic separation was performed on a Kinetex C18 column (5 μm, 50 mm × 4.6 mm I.D.) preceded by a C18 security guard cartridge (4.0 mm × 3.0 mm I.D.) both by Phenomenex (Torrance, CA, USA). Separation was attained using an isocratic elution with a flow rate of 0.5 mL.min-1. The column and tray temperature were set at 25 and 10°C, respectively. The mobile phase consisted of 0.5% formic acid in water and methanol (45:55 v/v). The run time of analysis was 3 min.
Mass spectrometry was performed on an LTQ XL linear ion trap instrument (Thermo Scientific, San Jose, CA, USA), coupled with heated electrospray ionization (HESI-II) probe operated in positive mode. After optimization, the parameters in the source were set as follows: source voltage 4.5 kV, source heater temperature 250°C, sheath gas flow 30 arb and auxiliary gas flow 10 arb. The mass spectrometer was operated in selected reaction monitoring (SRM) mode. The fragment ions m/z 180 → 163 and m/z 152 → 135 were used for quantification of memantine and amantadine, respectively. Thermo Fisher Xcalibur software was used for the analysis.