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Cc 3246

Manufactured by Lonza
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Sourced in United Kingdom

The CC-3246 is a laboratory equipment designed for a core function. The detailed specifications and intended use of this product are not available.

Automatically generated - may contain errors

Lab products found in correlation

Cc 3244, by Lonza (2 mentions) Cc 2580, by Lonza (2 mentions)

2 protocols using cc 3246

1

Differentiation of Primary Myoblasts

Cited 1 time
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Reagents were obtained from Sigma (Gillingham, UK) unless stated otherwise. Primary myoblasts obtained from healthy donors (CC‐2580; Lonza, Slough, UK) were maintained in-house in Skeletal Muscle Basal Medium‐2 (Lonza; CC‐3244 and CC‐3246) containing 0.1% human epidermal growth factor, 2% l‐glutamine, 10% fetal bovine serum (FBS), and 0.1% gentamicin/amphotericin‐B in the absence of GCs. Confluent myotubes were differentiated in Dulbecco’s modified Eagle’s medium (DMEM) containing 2% horse serum (HS) for 120 h. Media were replaced every 2–3 days as previously reported (16 (link)).
Webster J.M., Waaijenberg K., van de Worp W.R., Kelders M.C., Lambrichts S., Martin C., Verhaegen F., Van der Heyden B., Smith C., Lavery G.G., Schols A.M., Hardy R.S, & Langen R.C. (2023). 11β-HSD1 determines the extent of muscle atrophy in a model of acute exacerbation of COPD. American Journal of Physiology - Lung Cellular and Molecular Physiology, 324(4), L400-L412.
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2

Primary Myoblast Differentiation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents were obtained from Sigma (Gillingham, UK) unless stated otherwise. Primary myoblasts (CC‐2580; Lonza, Slough, UK) were maintained in house in Skeletal Muscle Basal Medium‐2 (Lonza; CC‐3244 and CC‐3246) containing 0.1% human epidermal growth factor, 2% l‐glutamine, 10% fetal bovine serum (FBS) and 0.1% gentamicin/amphotericin‐B in the absence of GCs. Confluent myotubes were differentiated in Dulbecco's modified Eagle's medium (DMEM) containing 2% horse serum (HS) for 120 h. Media were replaced every 2–3 days 25.
Hardy R.S., Doig C.L., Hussain Z., O'Leary M., Morgan S.A., Pearson M.J., Naylor A., Jones S.W., Filer A., Stewart P.M., Buckley C.D., Lavery G.G., Cooper M.S, & Raza K. (2016). 11β‐Hydroxysteroid dehydrogenase type 1 within muscle protects against the adverse effects of local inflammation. The Journal of Pathology, 240(4), 472-483.
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