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Jes re1x x band spectrometer

Manufactured by JEOL
Sourced in Japan

The JES-RE1X is an X-band spectrometer manufactured by JEOL. It is designed for electron paramagnetic resonance (EPR) spectroscopy, a technique used to study the properties of materials with unpaired electrons. The spectrometer operates at a frequency of approximately 9.5 GHz and is capable of detecting and analyzing the magnetic properties of these unpaired electrons.

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2 protocols using jes re1x x band spectrometer

1

Spectroscopic Characterization of Biomolecules

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Absorption spectra have been measured on a Shimadzu UV-2600 spectrometer or on a JASCO V-560 spectrometer, to both of which a temperature controller has been attached, using quartz cells with 1 mm or 10 mm path-length. Circular dichroism (CD) spectra have been measured on a JASCO J-720 spectropolarimeter. Electron paramagnetic resonance (EPR) spectrum has been measured on a JEOL JES-RE1X X-band spectrometer at 77 K.
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2

ROS Generation Measurement via ESR

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ROS generation in cells was measured using ESR, as previously described (19 (link)). Briefly, 106 cells were seeded on a glass cover slide (49.0×5.0×0.2 mm) and incubated overnight as previously described. Cells were treated with 0 or 1,000 µM TP4O for 15 min. ESR was measured in a respiration buffer containing 10 mM DMPO as the spin-trapping agent. All ESR spectra were obtained using a JES-RE1X X-band spectrometer (JEOL, Ltd., Tokyo, Japan).
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