Dmem f12 ham 1 1

Prostate adenocarcinoma (LNCaP), and bone metastatic (VCaP) cell lines were acquired from the Physical Sciences Oncology Network Bioresource Core Facility, through ATCC (Manassas, VA). These cell lines were cultured according to standard protocols, with subculturing at 80% confluence. LNCaP media was made of RPMI (Gibco;11835030), supplemented to match the ATCC formula (4.5 g/L glucose, 2.383 g/L HEPES, 0.11 g/L sodium pyruvate, 10% FBS). VCaP were cultured in DMEM F12: Ham 1:1 (Gibco; 11-320-033) containing 10% FBS. Media for all cell lines was supplemented with 100 mg/ml penicillin streptomycin (Gibco; 15-140-122).

RWPE-1, LNCaP, DU145, 22RV1, VCaP, and PC3 cell lines were obtained from the Physical Sciences Oncology Network Bioresource Core Facility, supported by ATCC (Manassas, VA).
All cell lines were cultured according to standard protocols, subculturing cells as they reached 80% confluency with the following media formulations: RWPE media was comprised of keratinocyte-specific media supplemented with EGF and bovine pituitary extract (Gibco; 17005042). RPMI (Gibco; 11835030) was supplemented to match the suggested formula by ATCC (4.5 g/liter glucose, 2.383 g/liter Hepes, and 0.11 g/liter sodium pyruvate) and 10% FBS (Corning; 35–010-CV). These media was used for LNCaP, 22RV1, and PC3. DU145 cells were cultured in DMEM supplemented with 10% FBS, and DMEM F12: Ham 1:1 (Gibco; 11–320-033) with 10% FBS was used for VCaP.
Cell lines MDAPCa2a and MDAPCa2b were a kind gift of Dr. Nora Navone (MD Anderson Cancer Center, Houston, TX). The identity of the received cell lines was verified by ATCC Human Cell STR testing services. These cell lines were grown in HPC1 media (Athena Enzyme Systems) supplemented with 10% FBS on standard cell culture T75 flasks coated with FCN Coating Mix (Athena Enzyme Systems) as per the manufacturer’s instructions.
All media formulations were supplemented with 100 µg/ml penicillin-streptomycin (Gibco; 15–140-122).