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The NP0343 is a laboratory equipment product offered by Thermo Fisher Scientific. It serves as a core function for laboratory applications, but a detailed and unbiased description is not available at this time.

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Lab products found in correlation

Np0321, by Cytiva (2 mentions) Wg1402, by Cytiva (2 mentions) Nxa931v, by Cytiva (2 mentions) G box imager, by Syngene (2 mentions) Nitrocellulose membrane, by Cytiva (1 mentions) Nupage lds sample buffer, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg, by Cytiva (1 mentions) Lds sample buffer, by Thermo Fisher Scientific (1 mentions) Na934v, by Cytiva (1 mentions)

2 protocols using np0343

1

Western Blot Protocol for Protein Analysis

Cited 1 time
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Western blots were performed as described previously59 (link). In brief, cells were lysed in NuPage LDS sample buffer (Invitrogen, NP0007) at 1 × 104 cells per µl. Proteins were resolved using NuPage Bis-Tris gel electrophoresis (Invitrogen, NP0343, NP0321 or WG1402) and transferred to nitrocellulose membranes (Amersham, 10600037). Secondary antibodies were peroxidase-conjugated anti-mouse IgG (Amersham, NXA931V) or anti-rabbit IgG (Amersham, NA934V). Peroxidase activity was detected using an ECL kit (Prometheus, 20-302B) and the Syngene G-Box imager. Primary antibodies are listed below.
Nassour J., Aguiar L.G., Correia A., Schmidt T.T., Mainz L., Przetocka S., Haggblom C., Tadepalle N., Williams A., Shokhirev M.N., Akincilar S.C., Tergaonkar V., Shadel G.S, & Karlseder J. (2023). Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis. Nature, 614(7949), 767-773.
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2

Western Blot Protein Analysis Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blots were performed as described32 (link). In brief, adherent and floating dead cells were lysed in NuPage LDS sample buffer (Invitrogen #NP0007) at 1 × 104 cells per μl. Proteins were resolved using NuPage Bis-Tris gel electrophoresis (Invitrogen #NP0343, #NP0321, or #WG1402) and transferred to nitrocellulose membranes (Amersham #10600037). Secondary antibodies were peroxidase-conjugated anti-mouse IgG (Amersham #NXA931V) or anti-rabbit IgG (Amersham #NA934V). Peroxidase activity was detected using an ECL kit (Prometheus #20–302B) and Syngene G-Box imager. Primary antibodies are listed below.
Nassour J., Radford R., Correia A., Fusté J.M., Schoell B., Jauch A., Shaw R.J, & Karlseder J. (2019). Autophagic cell death restricts chromosomal instability during replicative crisis. Nature, 565(7741), 659-663.
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