Human breast cancer cell lines (MCF7, Hs-578T, ZR-75-30, HCC1973) and human normal breast cell MCF-10A were purchased from the Chinese Academy of Sciences, Shanghai Institute of Biochemistry and Cell Biology (Shanghai, China). All cells were maintained in DMEM (Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, USA) and grown in humidified 37°C incubators supplied with 5% of CO2. The CO2 cell incubator purchased from Forma Scientific UK and FACS Calibri flow cytometer purchased from BD biosciences (USA). ABI7300 fluorescence quantitative PCR instrument was purchased from Applied Biosystems Inc.
Facs calibri flow cytometer
Manufactured by BD
Sourced in United Kingdom
The FACS Calibri flow cytometer is a laboratory instrument designed for the analysis and sorting of cells, particles, and molecules. It utilizes the principles of flow cytometry to rapidly detect and measure multiple physical and chemical characteristics of individual cells or particles as they flow in a fluid stream through a beam of light.
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3 protocols using facs calibri flow cytometer
1
Cell Line Cultivation and Characterization
2
Thyroid Cell Culture and Analysis
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Human normal thyroid cell line Nthy-ori3-1 (derived from human thyroid follicular epithelial normal cells) and human thyroid cancer cell lines (BCPAP, BHP5-16, TPC-1 and CGTH-W3) were purchased from Chinese Academy of Science (Shanghai, China). Cells were cultured in Roswell Park Memorial Institute (RPMI, KeyGEN, Nanjing, China) 1640 complete medium supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin in an incubator at 37°C with 5% CO2 and saturated humidity. The CO2 cell incubator purchased from Forma Scientific U.K. and FACS Calibri flow cytometer purchased from BD Biosciences (U.S.A.). ABI7300 fluorescence quantitative PCR instrument was purchased from Applied Biosystems Inc.
3
Cell Surface Marker Expression Quantification
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For measuring the expression status of cell surface markers, cells were harvested, the suspended inside staining buffer (PBS supplemented by 1% FBS and 0.1% NaN3), and incubated for 30 min using FITC-conjugated anti-human CD68 (BD Biosciences, USA) at 4 °C. Then the Analyses of FACS Calibri flow cytometer (BD Biosciences) were conducted on the cells from the Centre for Cytometry and Fluorescence Microscopy Complutense University of Madrid (Spain).
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