Bcl xl

MIN6 cells were seeded in six‐well plates and treated with the combination of IFN‐γ (100 ng/ml) and LIGHT (5 μg/ml) for 0, 0.5, 1, 12, 24 h or for the indicated times. In some experiments, cells were pre‐incubated with or without the NF‐κB inhibitor PDTC for 1 h and then treated with or without IFN‐γ (100 ng/ml) plus LIGHT (5 μg/ml) for 12 h. Antibodies against cytochrome c (BD Pharmingen, Franklin Lakes, NJ, USA), COX4 (BD Pharmingen), NF‐κB p65 (Beyotime Institute of Biotechnology), Bcl‐2 (Cell Signalling, Danvers, MA, USA), Bcl‐xL (Beyotime Institute of Biotechnology, Beijing, China), Bax (Beyotime Institute of Biotechnology), Bak (Beyotime Institute of Biotechnology), caspase‐9 (Beyotime Institute of Biotechnology), PARP (Beyotime Institute of Biotechnology), caspase‐3 (Cell Signalling, Danvers, MA, USA) and cleaved caspase‐3 (Cell Signalling, Danvers) were used to analyse the expression of proteins by Western blot as previously described 5.