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P ser724 ireα

Manufactured by Cell Signaling Technology
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P-Ser724 IREα is a monoclonal antibody that recognizes the phosphorylated form of the insulin receptor substrate (IRS) alpha protein at serine 724. This antibody is designed for use in various research applications such as Western blotting, immunoprecipitation, and other immunoassays to detect and study the phosphorylation of IRS-α.

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Lab products found in correlation

Pser9 gsk3β, by Cell Signaling Technology (2 mentions) Acrylamide sds page gel, by Bio-Rad (2 mentions) P ser455, by Cell Signaling Technology (2 mentions) P ireα, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions)

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P-IREα

2 protocols using p ser724 ireα

1

Adipose Tissue Protein Analysis

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Adipose tissues were homogenized on ice and protein concentrations were determined by Bradford assay as previously described (18) Adipose proteins were fractionated on 4–15% acrylamide SDS-PAGE gels (BioRad, Hercules, CA, USA) and transferred onto nitrocellulose membranes for the western analysis of p-Ser79 ACC, p-Ser455ACL, p-threonine308 kinase Akt/PKB, IR, IRS-1, GLUT4, p-Ser9 GSK3β, p-Ser724 IREα, ACC, ACL, XBP1, p-IREα, IREα, and GAPDH (Cell Signaling, Danvers MA). Proteins signals were quantified by Eagle Eye II Quantitation System (Stratagene, CA, USA).
Liu L., Wang Y., Wang J., Dong Y., Change S., Liu X., Lutfy K., Chen H., Friedman T.C., Jiang M, & Liu Y. (2018). Enhanced Hexose-6-Phosphate Dehydrogenase Expression in Adipose Tissue May Contribute to Diet-Induced Visceral Adiposity. International journal of obesity (2005), 42(12), 1999-2011.
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2

Adipose Tissue Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adipose tissues were homogenized on ice and protein concentrations were determined by Bradford assay as previously described (18) Adipose proteins were fractionated on 4–15% acrylamide SDS-PAGE gels (BioRad, Hercules, CA, USA) and transferred onto nitrocellulose membranes for the western analysis of p-Ser79 ACC, p-Ser455ACL, p-threonine308 kinase Akt/PKB, IR, IRS-1, GLUT4, p-Ser9 GSK3β, p-Ser724 IREα, ACC, ACL, XBP1, p-IREα, IREα, and GAPDH (Cell Signaling, Danvers MA). Proteins signals were quantified by Eagle Eye II Quantitation System (Stratagene, CA, USA).
Liu L., Wang Y., Wang J., Dong Y., Change S., Liu X., Lutfy K., Chen H., Friedman T.C., Jiang M, & Liu Y. (2018). Enhanced Hexose-6-Phosphate Dehydrogenase Expression in Adipose Tissue May Contribute to Diet-Induced Visceral Adiposity. International journal of obesity (2005), 42(12), 1999-2011.
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