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Nextseq 300 cycle mid output kits

Manufactured by Illumina

The NextSeq 300 cycle mid output kits are laboratory equipment designed for DNA sequencing applications. The kits provide the necessary reagents and consumables to perform DNA sequencing runs on the Illumina NextSeq 500/550 sequencing systems for up to 300 cycles, generating a moderate output of sequencing data.

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2 protocols using nextseq 300 cycle mid output kits

1

UMI-based Genomic DNA Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA from edited and unedited double transgenic 55 hpf embryos were extracted using the DNeasy kit (Qiagen). Samples were UMI tagged and PCR amplified using primers flanking the barcode as previously described23 (link). Sequencing adapters, sample indexes and flow cell adapters were incorporated by PCR, and libraries were quantified using the NEBNext Library Quant kit (NEB). Libraries were sequenced using NextSeq 300 cycle mid output kits (Illumina).
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2

UMI-based Genomic DNA Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA from edited and unedited double transgenic 55 hpf embryos were extracted using the DNeasy kit (Qiagen). Samples were UMI tagged and PCR amplified using primers flanking the barcode as previously described23 (link). Sequencing adapters, sample indexes and flow cell adapters were incorporated by PCR, and libraries were quantified using the NEBNext Library Quant kit (NEB). Libraries were sequenced using NextSeq 300 cycle mid output kits (Illumina).
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