Annexin 5 and fluorescein conjugate fitc annexin 5

Dimethyl sulfoxide (DMSO), Dulbecco’s phosphate-buffered saline (DPBS), Dulbecco’s Modified Eagle Medium (DMEM), tissue culture grade water, trypsin, and Hanks balanced salt solution (HBSS) were obtained from Lonza (Walkersville, MD, USA). Fluorescent probes, 5,5′, 6,6′-tetrachloro-1,1′, 3,3-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1), and propidium iodide (PI) were obtained from Sigma-Aldrich (St Louis, MO, USA). Fetal Bovine Serum (FBS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Annexin V and fluorescein conjugate (FITC annexin V) was purchased from Invitrogen (Carlsbad, CA, USA). The oxiselect ™ intracellular ROS Assay Kit (Green Fluorescence) was purchased from Cell Biolabs (San Diego, CA, USA). The XF Cell Mito Stress Kit was purchased from Agilent technologies (Santa Clara, CA, USA).

The Annexin V and fluorescein conjugate (FITC annexin V) was purchased from Invitrogen (Carlsbad, CA, USA). Propidium iodide (PI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 5,50, 6,60-tetrachloro-1,10, 3,3-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1), phenylmethylsulfonyl fluoride (PMSF) and trizma/hydrochloride (Tris/HCl) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified eagle medium (DMEM), dimethyl sulfoxide (DMSO), trypsin, dulbecco’s phosphate-buffered saline (DPBS), tissue culture-grade water, the ViaLight plus ATP assay kit and the Hanks balanced salt solution (HBSS) were obtained from Lonza (Walkersville, MD, USA). Fetal Bovine Serum (FBS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The Oxiselect™ Intracellular ROS assay kit (Green Fluorescence) was purchased from Cell Biolabs (San Diego, CA, USA). XF Cell Mito Stress Kit was purchased from Agilent Technologies (Santa Clara, CA, USA). The GSH/GSSH-Glo^™ assay kit and caspase-glo 3/7 were purchased from Promega, (Madison, WI, USA). The SOD activity assay kit (Abcam, Pretoria, SA, USA) was used. 7-Hydroxyflavanone was extracted from Galenia africana by BioPharm (Hamilton, New Zealand) and validated by means of high-performance liquid chromatography (HPLC)–ultraviolet using a reference standard obtained from Sigma-Aldrich (St. Louis, MO, USA).