Total RNA was extracted from U266 cells (n=3; treatment and control groups) using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.). RNA quality was checked using a Bioanalyzer 2200 instrument (Agilent Technologies, Inc.), and cDNA libraries were constructed for each pooled RNA sample using a NEBNext Ultra Directional RNA Library Prep Kit (Illumina, Inc.) according to the manufacturer's protocol. Tagged cDNA libraries were pooled in equal ratios and used for 150-bp paired-end sequencing in a single lane of an Illumina HiSeqXTen instrument (Illumina, Inc.).
Bioanalyzer 2200 instrument
Manufactured by Agilent Technologies
Sourced in United States
The Bioanalyzer 2200 instrument is a lab equipment product from Agilent Technologies. It is a microfluidics-based platform that enables automated, high-resolution analysis of DNA, RNA, and protein samples.
Automatically generated - may contain errors
Lab products found in correlation
Nebnext ultra directional rna library prep kit, by Illumina (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Hiseq x ten, by Illumina (1 mentions)
Nebnext small rna library prep kit, by Illumina (1 mentions)
Hiseq x ten platform, by Illumina (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
2 protocols using bioanalyzer 2200 instrument
1
Transcriptome Profiling of U266 Cells
2
RNA-seq Library Preparation and Sequencing
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Cellular RNA was extracted using Trizol (Invitrogen), with RNA quality confirmed using an Agilent 2200 machine. Samples that had an RNA integrity (RIN) score > 7.0 were then used for cDNA library preparation with the NEBNext Small RNA Library Prep Kit for Illumina. This RNA was first ligated to provided 3′ and 5′ adapters, after which first strand cDNA synthesis was performed. Index sequences and Illumina sequence adapters were then applied through index PCR, after which the library was purified, assessed for quality control with a Bioanalyzer 2200 instrument (Agilent, CA, USA), and sequences using a HiSeq X-ten platform (Illumina, CA, USA) with 150-bp paired-end reads.
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