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Boc gln ala arg amc

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Boc-Gln-Ala-Arg-AMC is a peptide substrate used in biochemical assays. It consists of the amino acid sequence Boc-Gln-Ala-Arg linked to the fluorescent reporter molecule 7-amino-4-methylcoumarin (AMC). This substrate can be used to measure the activity of proteases that cleave after the arginine residue.

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Lab products found in correlation

Luciferin luciferase based atp determination kit, by Thermo Fisher Scientific (2 mentions) Supersignal west femto chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) 3 3 diaminobenzidine tetrahydrochloride dab, by Thermo Fisher Scientific (1 mentions) Recombinant dnase 1, by Roche (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Caerulein, by Merck Group (1 mentions) Leupeptin, by Roche (1 mentions) Aprotinin, by Roche (1 mentions) Collagenase p, by Roche (1 mentions) Collagenase, by Worthington (1 mentions) Protein determination reagent, by Bio-Rad (1 mentions) Dna ligase, by Takara Bio (1 mentions) Protein markers, by Solarbio (1 mentions) E coli bl21 de3, by Transgene (1 mentions) E coli trans t1, by Transgene (1 mentions) Hrv 3c protease, by Takara Bio (1 mentions) Dna ladder, by Solarbio (1 mentions)

Spelling variants of this product

Ala-AMC (4 citations) Boc-Gln-Ala-Arg-AMC · HCl (3 citations)

4 protocols using boc gln ala arg amc

1

Pancreatic acinar cell isolation

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Caerulein (#C9026), trypsin inhibitor (SBTI) (#T9003), protease (#P5147) were purchased from Sigma Aldrich (St Louis, MO), collagenase (CLSPA) was purchased from Worthington Biochemical Co. (Lakewood, NJ), collagenase P (11213857001), DNase I recombinant (04716728001), leupeptin and aprotinin were purchased from Roche Diagnostics (Indianapolis, IN), bovine albumin serum fraction V (BSA) from MP Biomedicals (Solon, OH), Phadebas Amylase Assay kit was purchased form Magle Life Sciences (Cambridge, MA), Boc-Gln-Ala-Arg-AMC from BachemAG (Bubendrof, Switzerland), Dulbecco’s minimal essential medium (DMEM)/high glucose from HyClone (Logan, Utah), TRIzol, and 3,3-diaminobenzidine tetrahydrochloride (DAB) from Thermo Scientific (Rockford, IL). Protein determination reagent was purchased from Bio-Rad Life Science Research (Hercules, CA). Supersignal West Femto Chemiluminescent Substrate was purchased from Thermo Fisher Scientific (Rockford, IL).
Xia D., Halder B., Godoy C., Chakraborty A., Singla B., Thomas E., Shuja J.B., Kashif H., Miller L., Csanyi G, & Sabbatini M.E. (2019). NADPH oxidase 1 mediates caerulein-induced pancreatic fibrosis in chronic pancreatitis. Free radical biology & medicine, 147, 139-149.
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2

Recombinant Protein Expression in E. coli

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E. coli TransT1 and E. coli BL21 (DE3) were obtained from TransGen (Beijing, China). DNA polymerase, DNA ladder, and protein markers were from Solarbio (Beijing, China). Exonuclease, HRV 3C Protease, and DNA ligase were from Takara (Dalian, China). Boc–Gln–Ala–Arg–AMC was from Bachem (Bubendorf, Switzerland). 6–isopropyl–β–d–thiogalactopyranoside (IPTG) was from Sigma–Aldrich (St. Louis, MO, USA). His antibody was from Beyotime (Shanghai, China). All other chemicals were of the highest reagent grade commercially available.
Ye X., Ling X., Wu M., Bai G., Yuan M, & Rao L. (2023). Improving Soluble Expression of SARS-CoV-2 Spike Priming Protease TMPRSS2 with an Artificial Fusing Protein. International Journal of Molecular Sciences, 24(13), 10475.
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3

Pancreatic Enzyme and ATP Assays

Cited 1 time
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Animal serum amylase and lipase activities were determined by Antech Diagnostics (Irvine, CA) Custom Service. Active trypsin in pancreatic tissue homogenates was measured using Boc-Gln-Ala-Arg-AMC (Bachem Americas Inc, Torrance, CA) as a substrate by a fluorogenic assay as described previously [31 (link),32 (link)]. ATP level in pancreatic tissue extracts was measured according to the manufacturer’s instructions using luciferin/luciferase-based ATP determination kit (#A22066, Thermo Fisher Scientific, Grand Island, NY).
Yuan J., Chheda C., Tan G., Elmadbouh O, & Pandol S.J. (2022). Protein kinase D: a therapeutic target in experimental alcoholic pancreatitis. Biochimica et biophysica acta. Molecular basis of disease, 1868(11), 166486.
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4

Pancreatic Enzyme and Metabolite Assays

Cited 2 times
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Animal serum amylase and lipase activities were determined by Antech Diagnostics (Irvine, CA) Custom Service. Amylase activity in isolated pancreatic acini and the incubation medium was determined by using a commercial kit (Phaebadas kit; Pharmacia Diagnostic, Rochester, NY) as described [31 (link)]. Amylase secretion was calculated as the percent total cellular amylase released into the medium [medium / (medium + cells)]. Caspase 3 activities in pancreatic tissue homogenates were measured using a fluorogenic assay with substrates specific for caspase-3 (Ac-DEVDAMC) as we described previously [25 (link),26 (link)]. Active trypsin in pancreatic tissue homogenates was measured using Boc-Gln-Ala-Arg-AMC (Bachem Americas Inc, Torrance, CA) as a substrate by a fluorogenic assay as described previously [31 (link),42 (link)]. Cathepsin B activities in pancreatic homogenates were measured as described previously [31 (link)] using fluorogenic cathepsin B substrate III: Z-Arg-Arg-AMC, 2HCl (Millipore Sigma, Burlington, Massachusetts). ATP level in pancreatic tissue extracts was measured according to the manufacturer’s instructions using luciferin/luciferase-based ATP determination kit (#A22066, Thermo Fisher Scientific, Grand Island, NY).
Yuan J., Chheda C., Piplani H., Geng M., Tan G., Thakur R, & Pandol S.J. (2020). Pancreas-specific deletion of protein kinase D attenuates inflammation, necrosis, and severity of acute pancreatitis. Biochimica et biophysica acta. Molecular basis of disease, 1867(1), 165987.
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