Serum creatinine

Manufactured by Arbor Assays

Serum creatinine is a laboratory test used to measure the amount of creatinine in the blood. Creatinine is a waste product produced by the body's muscles and is filtered out of the blood by the kidneys. This test is commonly used to evaluate kidney function.

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Lab products found in correlation

Emax plus reader, by Molecular Devices (1 mentions) Anti dsdna antibodies, by Alpha Diagnostic (1 mentions) Radioimmunoassay, by DiaSorin (1 mentions)

Close spelling variants of this product

Creatinine Serum Detection Kit Detect X Serum Creatinine Detection Kit DetectX® Serum Creatinine kit Creatinine

2 protocols using serum creatinine

Serum Biomarker Profiling Protocol

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Sera were assessed for anti-nuclear antibodies (ANA; Alpha Diagnostic International, San Antonio, TX), anti-dsDNA antibodies (Alpha Diagnostic Int’l), blood urea nitrogen (BUN; Arbor Assays, Ann Arbor, MI), and serum creatinine (Arbor Assays) per manufacturers’ protocols. For ANA and anti-dsDNA assays, sera were measured in duplicate at a 1:100 dilution in a 96-well plate format, and the HRP-coupled secondary Ab was goat anti mouse IgG (H and L). Negative and positive control sera, as well as 5 point calibration curve samples, provided by the manufacturer, were run concurrently with the unknown samples. Sera were diluted 1:10 for BUN assays and 1:30 for creatinine assays, per manufacturers’ protocols. Sera were run in duplicate alongside a 5 (creatinine) or 7 (BUN) point standard curve. All assays were read at 450 nm using an Emax Plus Reader (Molecular Devices). Unknowns were compared with a calibration curve containing five dilution points on each assay plate. In all cases, the coefficient of determination for the standard curve (r²) was ≥0.98.

Munroe M.E., Anderson J.R., Gross T.F., Stunz L.L., Bishop G.A, & James J.A. (2021). Epstein-Barr Functional Mimicry: Pathogenicity of Oncogenic Latent Membrane Protein-1 in Systemic Lupus Erythematosus and Autoimmunity. Frontiers in Immunology, 11, 606936.

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Pig Model of Renal Dysfunction

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All procedures were approved by the Institutional Animal Care and Use Committee. Twenty-one female domestic pigs (initially weighing 25–35kg) were randomized to three groups: Normal, RAS and ARAS, which underwent imaging studies after 10 weeks of observation.
In vitro studies were subsequently performed to measure total cholesterol and low-density lipoprotein (LDL) in plasma (Roche), plasma renin activity (Radioimmunoassay, DiaSorin, Stillwater, MN), and serum creatinine (Arbor Assays, Ann Arbor, MI). After completion of all studies, the pigs were euthanized with sodium pentobarbital (100 mg·kg⁻¹ IV, Fort Dodge Laboratories). Kidneys were sectioned and immediately shock-frozen in liquid nitrogen and stored at −80°C, pres erved in formalin or prepared for micro-computed tomography (micro-CT).

Sun D., Eirin A., Ebrahimi B., Textor S.C., Lerman A, & Lerman L.O. (2016). Early atherosclerosis aggravates renal microvascular loss and fibrosis in swine renal artery stenosis. Journal of the American Society of Hypertension : JASH, 10(4), 325-335.

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