Anti icp8

Manufactured by Santa Cruz Biotechnology

Sourced in Germany

Anti-ICP8 is a laboratory product developed by Santa Cruz Biotechnology. It is an antibody that specifically recognizes the ICP8 protein. ICP8 is a single-stranded DNA-binding protein that plays a crucial role in the replication and recombination processes of certain viruses. The primary function of Anti-ICP8 is to facilitate the detection and study of the ICP8 protein in various experimental settings.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using anti icp8

Comprehensive Antibody Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: anti-SLFN5 (Sigma-Aldrich; Cat.HPA017760; Lot.B96361), anti-SLFN11 (Novus Biologicals; Cat.NBP1–92368; Lot.H96783), anti-PML (Bethyl Laboratory; Cat.A301–167A, Santa Cruz; Cat.sc-966; Lot.H1413), anti-IFI16 (Santa Cruz; Cat.sc-8023; Lot.C1312), anti-ATRX (Santa Cruz; Cat.sc-15408), anti-DNA-PKcs (Santa Cruz; Cat.sc-5282; Lot.G280), anti-SUMO2+3 (Abcam; Cat.ab3742; Lot.GR8249–1), anti-RNAP II (Santa Cruz; Cat.sc-56767), anti-GFP rabbit (Abcam; Cat.ab290; Lot.GR3251545–1), anti-GFP mouse (Millipore; Cat.MAB2510; Lot.2512480), anti-RAD50 (GeneTex; Cat.GTX70228; Lot.40186), anti-V5 (Santa Cruz; Cat.sc-271944; Lot.E2217), anti-HA (Abcam; Cat.ab9110; Lot.GR3217183–2), anti-GAPDH (GeneTex; Cat.GTX100118; Lot.42158), anti-α-Tubulin (Santa Cruz; Cat.sc-69969; Lot.DO412), anti-β-Actin (Sigma-Aldrich; Cat.a5441; Lot.064M4789V), anti-KU70 (Abcam; Cat.ab83501; Lot.GR3176811–2), anti-Histone H3 (Abcam; Cat.ab1791; Lot.GR3198176–1), anti-ICP0 (Santa Cruz; Cat.sc-53070; Lot. A0313), anti-ICP8 (gifted from David M. Knipe), anti-TK (Santa Cruz; Cat.sc-28037; Lot.K1813), anti-VP21 and anti-gD (gifted from Gary H. Cohen), anti-IE1/IE2 (Virusys; Cat.P1215; Lot.A1345070), anti-UL44 (Virusys; Cat.ca006–100; Lot.C1034151), adenovirus late protein antibody staining Hexon, Penton and Fiber (gift from James M. Wilson), and anti-DBP (gift from Arnold J. Levine).

Kim E.T., Dybas J.M., Kulej K., Reyes E.D., Price A.M., Akhtar L.N., Orr A., Garcia B.A., Boutell C, & Weitzman M.D. (2021). Comparative proteomics identifies Schlafen 5 (SLFN5) as a herpes simplex virus restriction factor that suppresses viral transcription. Nature microbiology, 6(2), 234-245.

+ Open protocol

+ Expand

Antibody Characterization for SLFN5, SLFN11, and DNA Repair

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: anti-SLFN5 (Sigma-Aldrich; Cat.HPA017760; Lot.B96361), anti-SLFN11 (Novus Biologicals; Cat.NBP1-92368; Lot.H96783), anti-PML (Bethyl Laboratory; Cat.A301-167A, Santa Cruz; Cat.sc-966; Lot.H1413), anti-IFI16 (Santa Cruz; Cat.sc-8023; Lot.C1312), anti-ATRX (Santa Cruz; Cat.sc-15408), anti-DNA-PKcs (Santa Cruz; Cat.sc-5282; Lot.G280), anti-SUMO2+3 (Abcam; Cat.ab3742; Lot.GR8249-1), anti-RNAP II (Santa Cruz; Cat.sc-56767), anti-GFP rabbit (Abcam; Cat.ab290; Lot.GR3251545-1), anti-GFP mouse (Millipore; Cat.MAB2510; Lot.2512480), anti-RAD50 (GeneTex; Cat.GTX70228; Lot.40186), anti-V5 (Santa Cruz; Cat.sc-271944; Lot.E2217), anti-HA (Abcam; Cat.ab9110; Lot.GR3217183-2), anti-GAPDH (GeneTex; Cat.GTX100118; Lot.42158), anti-α-Tubulin (Santa Cruz; Cat.sc-69969; Lot.DO412), anti-β-Actin (Sigma-Aldrich; Cat.a5441; Lot.064M4789V), anti-KU70 (Abcam; Cat.ab83501; Lot.GR3176811-2), anti-Histone H3 (Abcam; Cat.ab1791; Lot.GR3198176-1), anti-ICP0 (Santa Cruz; Cat.sc-53070; Lot. A0313), anti-ICP8 (gifted from David M. Knipe), anti-TK (Santa Cruz; Cat.sc-28037; Lot.K1813), anti-VP21 and anti-gD (gifted from Gary H. Cohen), anti-IE1/IE2 (Virusys; Cat.P1215; Lot.A1345070), anti-UL44 (Virusys; Cat.ca006-100; Lot.C1034151), adenovirus late protein antibody staining Hexon, Penton and Fiber (gift from James M. Wilson), and anti-DBP (gift from Arnold J. Levine).

+ Open protocol

+ Expand

Indirect Immunofluorescence Analysis of Transfected or Infected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Indirect immunofluorescence (IF) analysis of transfected or infected cells was done as described [14 (link)]. For plasmid transfection, the Effectene Transfection Reagent was used. For virus infection, HeLa cells were infected at the indicated MOI. In infected cells, binding of antibodies to the HSV1 Fc-receptor like proteins gE/gI was blocked overnight at 4 °C with human IgG (200 μg/ml) and 10 % FCS in PBS [16 (link)]. The mouse monoclonal antibodies anti-myc [9E10] (Santa Cruz), anti-ICP8 (provided by R. Heilbronn, Charité Universitätsmedizin Berlin CCM, Berlin, Germany), anti-Asna1/TRC40 ([M03], Klon 2H3 Abnova) and rabbit polyclonal antibodies anti-pUL34 [9 (link)], anti-Calreticulin (Sigma), and anti-Giantin (Abcam) were used as primary reagents. Goat anti-rabbit or anti-mouse antibodies coupled to Alexa488 or Alexa594 (all Invitrogen) were used as secondary reagents. Cells were examined using a Leica confocal laser scanning microscopes TCS SP5 and LSM710. Images were recorded using the Leica Application Suite AF6000 Software and processed using Adobe Photoshop.

Ott M., Marques D., Funk C, & Bailer S.M. (2016). Asna1/TRC40 that mediates membrane insertion of tail-anchored proteins is required for efficient release of Herpes simplex virus 1 virions. Virology Journal, 13, 175.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!