The DNA synthesis rate was assessed using the Beyo-ClickTM EdU kit (Beyotime). The cells were incubated with 10 μM EdU for 2 h, followed by staining according to the manufacturer’s instructions. The absorbance values of all wells at 370 nm were determined with an enzyme immunoassay instrument (Thermo Fisher Scientific).
Enzyme immunoassay instrument
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Enzyme Immunoassay Instrument is a versatile laboratory equipment used for the detection and quantification of specific analytes in a sample. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to measure the presence and concentration of target molecules, such as proteins, hormones, or antibodies, in a given sample. The instrument automates the ELISA process, providing consistent and reliable results for a wide range of applications in clinical diagnostics, research, and pharmaceutical development.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using enzyme immunoassay instrument
1
Quantifying DNA Synthesis via EdU Assay
2
Cell Proliferation, Colony Formation, and Migration Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the in vitro cell proliferation assay, the CCK-8 assay was performed using the CCK-8 kit (Bioss, Beijing, China) according to the manufacturer’s protocol. An enzyme immunoassay instrument (Thermo Fisher Scientific, USA) was used to detect the OD value at 450 nm. An in vitro colony formation assay was performed to determine whether colony formation was possible in vitro, and the procedure was performed as previously described [68 (link), 69 (link)]. Finally, the migration ability of the cells was determined using the Transwell chamber system (BD Biosciences, BD Biosciences, Franklin Lakes, NJ, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!