Polystyrene plates

Manufactured by BD

Polystyrene plates are a type of laboratory equipment used for various applications. They are flat, circular dishes typically made of transparent polystyrene plastic. Polystyrene plates are designed to provide a stable and sterile surface for cell culture, sample preparation, and other experimental purposes.

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Lab products found in correlation

Proteinase k, by Thermo Fisher Scientific (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Dnase 1, by Roche (1 mentions) Cellulase, by Merck Group (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Polystyrene 96-well plates (4 citations) 24‐well polystyrene microtiter plates (2 citations) Polystyrene culture plates (2 citations) Polystyrene nonpyrogenic 96-well plates (2 citations) 96-well polystyrene microtiter plates (2 citations) 96-well polystyrene flat-bottom microtiter plates (2 citations) Polystyrene microtiter plates (2 citations) Flat-bottom 96-well polystyrene plates (2 citations) 24-well medical-grade polystyrene plates (2 citations) 12-well polystyrene plate (2 citations)

2 protocols using polystyrene plates

Evaluating Dispersants' Impact on Francisella Biofilms

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The effect of dispersants was evaluated by using 24 h and 48 h-old biofilms of F. novicida and F. philomiragia biofilms, respectively. F. novicida biofilms were grown in 96-well polystyrene plates (Falcon^®) starting from an inoculum of 1x10⁷ CFU/mL (2x10⁶ bacteria/well), while for F. philomiragia 5x10⁷ CFU/m (10⁷ bacteria/well) were used. After one (F. philomiragia) or two (F. novicida) gentle PBS washes, the MMH was replaced with 200 μL fresh medium supplemented with increasing concentrations of dispersing agents including DNase I (Roche), EDTA (Sigma-Aldrich), chitinase from Streptomyces griseus (E.C. 3.2.1.14) (Sigma-Aldrich), cellulase (Sigma-Aldrich), proteinase K (Invitrogen) or Dispersin B (Kane Biotech Inc. Canada). Microtiter plates were incubated at 37°C for 24 h under 5% CO₂ and biofilm was quantified as described elsewhere. Data were expressed as percentage of remaining biofilm calculated in comparison to untreated biofilm under the same experimental conditions.

Siebert C., Villers C., Pavlou G., Touquet B., Yakandawala N., Tardieux I, & Renesto P. (2020). Francisella novicida and F. philomiragia biofilm features conditionning fitness in spring water and in presence of antibiotics. PLoS ONE, 15(2), e0228591.

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Macrophage Phagocytosis Inhibition Assay

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RAW264.7 Macrophages were cultured in DMEM (Life Technologies) + 10% FBS (Life Technologies) +1% penstrep (Life Technologies) media in 12 well tissue culture treated polystyrene plates (BD Falcon) until 75% confluent at which point treatments (n=3) were applied (controls, 0.1uM, 0.5uM, and 1uM Lat A micelles, 1uM Free Lat A). Treatments were incubated with cells at 37°C for 2 hours, and subsequently 15 μg/mL pHrodo red dextran dye (Thermo Fisher) was added to each well for 15 minutes. After this incubation, cells were washed with PBS and cell staining buffer and removed from 12 well plates by cell scraping method. Control groups included PBS treatment with no pHrodo chase, PBS treatment with a pHrodo chase, and cells treated with 1 μM Lat A micelles with no pHrodo chase. These groups were used to normalize flow data. Data was normalized as a percentile of uptake inhibition between the maximum uptake (pHrodo chase) and minimum uptake (PBS treatment).

Stack T., Vahabikashi A., Johnson M, & Scott E. (2018). Modulation of Schlemm’s Canal Endothelial Cell Stiffness via Latrunculin Loaded Block Copolymer Micelles. Journal of biomedical materials research. Part A, 106(7), 1771-1779.

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