Cell lines were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA (Wako Pure Chemical Industries).(22 (link)) After washing with phosphate-buffered saline (PBS), the cells were treated with primary antibodies (1 μg/mL) for 30 min at 4°C, followed by treatment with Oregon green-conjugated anti-mouse IgG (Life Technologies), Alexa Fluor 488 conjugated anti-mouse IgG (Cell Signaling Technology, Danvers, MA), or Alexa Fluor 488 conjugated anti-rat IgG (Cell Signaling Technology). Fluorescence data were collected using a FACS Calibur flow cytometer (BD Biosciences, Braintree, MA) or a Cell Analyzer EC800 (Sony, Tokyo, Japan).
Oregon green conjugated anti mouse igg
Manufactured by Thermo Fisher Scientific
Oregon green-conjugated anti-mouse IgG is a secondary antibody reagent. It is used to detect the presence of mouse immunoglobulin G (IgG) in various immunological assays and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Ec800 cell analyzer, by Sony (3 mentions)
Ethylene diamine tetraacetic acid (edta), by Fujifilm (2 mentions)
Trypsin, by Fujifilm (2 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Alexa fluor 488 conjugated anti rat igg, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 conjugated anti mouse igg, by Cell Signaling Technology (1 mentions)
Sa3800 cell analyzer, by Sony (1 mentions)
Ec800, by Sony (1 mentions)
Fitc conjugated anti human igg, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Nacalai Tesque (1 mentions)
4 protocols using oregon green conjugated anti mouse igg
1
Cell Harvesting and Immunofluorescence Analysis
2
Cell Harvesting and Immunofluorescence Assay
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Cell lines were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA (Wako Pure Chemical Industries Ltd.)6 (link). After washing with phosphate buffered saline (PBS), the cells were treated with primary antibodies (1 μg/ml) for 30 min at 4°C, followed by treatment with Oregon green-conjugated anti-mouse IgG (Life Technologies Corp.). Fluorescence data were collected using a Cell Analyzer EC800 (Sony Corp., Tokyo, Japan).
3
HER2 Expression Quantification by Flow Cytometry
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Cells were harvested by brief exposure to 0.25% trypsin/1-mM ethylenediaminetetraacetic acid (EDTA) (Nacalai Tesque, Inc.). After washing with 0.1% bovine serum albumin (BSA)/phosphate-buffered saline, the cells were treated with 1 μg/mL of anti-HER2 (clone H2Mab-77) for 30 minutes at 4°C and then with Oregon green-conjugated anti-mouse IgG (1:1000 diluted; Thermo Fisher Scientific, Inc.). Fluorescence data were collected using EC800 or SA3800 Cell Analyzers (Sony Corp.).
4
Cell Staining Using LpMab-7 and chLpMab-7
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Cells were harvested by brief exposure to 0.25% Trypsin/1 mM EDTA (Nacalai Tesque, Inc.). After washing with PBS, the cells were treated with LpMab-7 and chLpMab-7 (1 μg/ml) for 30 min at 4°C followed by incubation with Oregon green-conjugated anti-mouse IgG or FITC-conjugated anti-human IgG (Thermo Fisher Scientific Inc.). Fluorescence data were collected using a Cell Analyzer EC800 (Sony Corp., Tokyo, Japan).
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