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Alpha 300 r

Manufactured by Oxford Instruments

The Alpha 300 R is a high-performance Raman microscope designed for materials analysis. It features a compact and modular design, providing users with a versatile and customizable platform for a wide range of Raman spectroscopy applications.

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2 protocols using alpha 300 r

1

Raman Microscopy of Sample Preparation

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The aforementioned sample preparation procedure was repeated for the Raman microscopy measurements, although without the addition of R6G in order to prevent interfering fluorescence. The Raman signal was measured on a confocal Raman microscope (Witec, Alpha 300 R) coupled to a CMOS camera (Andor, Newton EMCCD, DU970P-BVF-355). The laser wavelength used was 532 nm with a grating of 600g mm-1 . Each spectrum was averaged 25 times with an integration time of 30 s.
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2

Raman Spectroscopy for Material Analysis

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Raman spectroscopy measurements are carried out using a confocal Raman microscope (Witec, Alpha 300R) with an EMCCD detector (Andor, Newton, DU970P-BVF-355). Excitation is performed with a 532 nm laser while spectra are collected in a spectrometer, equipped with a diffraction grating with a groove density of 600 grooves mm−1. The spectra are each 20 times averaged with an integration time of 1 s.
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