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2 protocols using phosphate buffer

1

Antioxidant and Antimicrobial Evaluation of Natural Extracts

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L-ascorbic acid, bovine serum albumin, 2-2-diphenyl-1-picrylhydrazyl, gum arabic, 2-thiobarbituric acid, phosphate buffer saline (PBS), and cyclohexanone were purchased from Sigma Aldrich Co. St. Louis, MO, USA. Sodium hydroxide, ethanol, citric acid, hydrochloric acid, nitric acid, Tween 80, phosphate buffer, and ammonium sulfate were purchased from Loba Chemie Pvt Ltd. Navi Mumbai, India. Standard antibiotics (ciprofloxacin), Muller Hinton Agar, nutrient agar, and nutrient broth were procured from Hi-Media Private Limited, Mumbai, India. Gram-positive and Gram-negative bacterial strains, i.e., Staphylococcus aureus (MTCC 3160) and Escherichia coli (MTCC 443), were obtained from the Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India. Analytical grade chemicals and acid-washed glassware were used throughout the experiments.
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2

Standardized Analytical Reagents Procurement

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HPLC grade standards include caffeic acid, syringic acid, p-coumaric acid, rutin, L-ascorbic acid, BSA (bovine serum albumin), DPPH (2-2-diphenyl-1-picrylhydrazyl), and phosphate buffer saline (PBS) were procured from Sigma Aldrich Co. St. Louis, USA. Other 'Analytical Reagent grade chemicals including sodium hydroxide, ethanol, citric acid, hydrochloric acid, nitric acid, phosphate buffer, and diclofenac sodium were procured from Loba Chemie Pvt Ltd. Navi Mumbai, India. For microbiological experiments, standard antibiotics (ciprofloxacin), Mueller Hinton Agar (MHA), nutrient agar, and nutrient broth were purchased from Hi-Media Pvt. Ltd., Mumbai, India. Pathogenic microbial strains Staphylococcus aureus (MTCC 3160) and Escherichia coli (MTCC 443), were procured from the Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India. For the accomplishment of the research, deionized water and aqua-regia washed class 'A' glassware was used.
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