Mice were administered 0.75 μg of recombinant IL-33 (BioLegend, San Diego, CA) or PBS via intraperitoneal injection daily beginning at 3 days before amebic challenge up until 1 day before harvest. For sST2 experiments, mice were treated with 5ug/mouse of ST2-Fc fusion (R&D Systems, Catalog # 1004-MR-050) or Fc control for four days (day −1, day 0, day+1, and day+2 of amebic challenge) intraperitoneally. For the Chil3 treatment, mice were administered 6 doses (on day −2, −1, 0, +1, +2 +3) of recombinant Chil3 (5ug/dose) (R&D Systems) via intraperitoneal injection.
Recombinant chil3
Manufactured by R&D Systems
Recombinant Chil3 is a human chitinase-3-like protein 1 (CHI3L1) produced in a mammalian cell expression system. CHI3L1 is a secreted glycoprotein that lacks enzymatic chitinase activity but is involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant chil3
1
Modulating Amebic Infection Using IL-33, sST2, and Chil3
2
Modulation of Amebic Infection in Mice
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Mice were administered 0.75 µg of recombinant IL-33 (BioLegend, San Diego, CA) or PBS via intraperitoneal injection daily beginning at 3 days before amebic challenge up until 1 day before harvest. For sST2 experiments, mice were treated with 5 ug/mouse of ST2-Fc fusion (R&D Systems, Catalog #1004-MR-050) or Fc control for four days (day–1, day 0, day+1, and day+2 of amebic challenge) intraperitoneally. For the Chil3 treatment, mice were administered 6 doses (on day–2, –1, 0, +1, +2, +3) of recombinant Chil3 (5ug/dose) (R&D Systems) via intraperitoneal injection.
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