Cells were harvested in lysis buffer, as described previously (18 (link)), and subjected to Western blotting. Anti-MSK1, anti-pMSK1, anti--β-catenin, anti-pS552-β-catenin, anti-ERK, anti-pERK, anti-S6, anti-pS6, anti-AKT and anti-pS473-AKT antibodies were from Cell Signaling (Boston, MA). Anti-β-actin antibody was purchased from Sigma (St. Louis, MO) and used as a loading control.
Anti pmsk1
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-pMSK1 is a primary antibody product from Cell Signaling Technology that detects the phosphorylated form of the Mitogen- and Stress-activated protein Kinase 1 (MSK1). MSK1 is a serine/threonine protein kinase that is activated in response to various extracellular stimuli.
Automatically generated - may contain errors
Lab products found in correlation
Anti msk1, by Cell Signaling Technology (2 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti erk, by Cell Signaling Technology (1 mentions)
Anti β catenin, by Cell Signaling Technology (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Anti ps473 akt, by Cell Signaling Technology (1 mentions)
Anti ps552 β catenin, by Cell Signaling Technology (1 mentions)
Anti p erk, by Cell Signaling Technology (1 mentions)
Anti s6, by Cell Signaling Technology (1 mentions)
Anti ps6, by Cell Signaling Technology (1 mentions)
Anti bax, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Anti cyclin d1, by Cell Signaling Technology (1 mentions)
Anti p rsk2, by Cell Signaling Technology (1 mentions)
Anti rsk2, by Cell Signaling Technology (1 mentions)
Anti erk2, by Cell Signaling Technology (1 mentions)
Anti p erk1 2, by Cell Signaling Technology (1 mentions)
2 protocols using anti pmsk1
1
Immunoblotting Analysis of Cellular Signaling
2
Protein Expression Profiling by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The extracted protein from cells was separated on 10% sodium dodecyl sulfate polyacrylamide gels and transferred to nitrocellulose membranes at 100 mA for 2 h. The membranes were blocked in skimmed milk for 1 h at room temperature and overnight at 4°C in anti-CyclinD1, anti-Bcl-2, anti-Bax (1:1,000) and anti-ERK2, anti-p-ERK1/2, anti-RSK2, anti-p-RSK2, anti-MSK1 and anti-p-MSK1 (1:800; Cell Signaling Technology, Danvers, MA, USA), respectively, before they were conjugated with the secondary antibody. Signals were observed using Cano Scan (LiDE110, Tokyo, Japan).
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