Secondary antimouse and antirabbit antibodies conjugated with hrp

CIS, DOC, irinotecan (IRINO), HCQ, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma Aldrich, St. Louis, MO, USA. Nutlin-3 (NUT) was obtained from Tocris Bioscience, Bristol, UK. Antibodies against p53 and p16 were purchased from Santa Cruz Biotechnology, Dallas, TX, USA. Antibodies against: E-cadherin, N-cadherin, cyclin B, p-Rb, p-cdc2, SOX-2, and Nanog were procured from Cell Signaling Technology, Danvers, MA, USA. Antibodies against p21 were bought from Sigma Aldrich, St. Louis, MO, USA. Antibodies against p-S6 (Ser235/236) and p62 were purchased from BD Transduction Laboratories™, Lexington, KY, USA. Antibodies against GAPDH were procured from Millipore, Temecula, CA, USA and ALDH1A from LifeSpan Biosciences, Seattle, WA, USA. Secondary antimouse and antirabbit antibodies conjugated with HRP were obtained from Vector Laboratories, Burlingame, CA, USA.

Unless otherwise specified, chemicals and reagents were purchased from Sigma Aldrich. Antibodies against: p53 (DO-1) and p21Cip1 (C-19) were purchased from Santa Cruz Biotechnology, Ki-67 from Dako, PARP-1 from Enzo, p-p53 (Ser15), p-S6 (Ser235/236), and Nanog from Cell Signaling, p62 from Transduction Laboratories^TM, γ-H2A.X (Ser 139) from Abcam, H2A.X and GAPDH from Millipore. Secondary anti-mouse and anti-rabbit antibodies conjugated with HRP were obtained from Vector Laboratories, and ECL reagents from Thermo Scientific. LysoTracker^® Red DND-99, DilC₁₂(3) (Dil), secondary antibodies conjugated with AlexaFluor 488 or AlexaFluor 555 were purchased from Molecular Probes. Mounting medium, protease inhibitor cocktail and phosphatase inhibitor cocktail were obtained from Roche Diagnostics. 7-AAD, Matrigel Matrix, Matrigel Matrix Growth Factor Reduced, FITC mouse anti-human CD24, FITC mouse IgG2a, κ isotype control, AlexaFluor^® 700 mouse IgG2b, κ isotype control, Alexa Fluor^®700 mouse anti-human CD44 were obtained from BD Pharmingen^TM, APC mouse IgG1 isotype control, APC mouse anti-human CD133/1 (AC133) were purchased from Miltenyi Biotec. ELISA kits for human vascular endothelial growth factor (VEGF), and human interleukin-8 (IL-8), neutralizing antibodies against human VEGF and corresponding isotype control antibodies were procured from R&D Systems.