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Epigenase m6a methylase activity inhibition assay kit

Manufactured by Epigentek
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Sourced in United States

The Epigenase m6A methylase activity/inhibition assay kit is a laboratory tool designed to measure the activity or inhibition of m6A methylase enzymes. The kit provides a quantitative, non-radioactive method for detecting and analyzing the methylation of target substrates by m6A methylase enzymes.

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Lab products found in correlation

Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions) Tri reagent, by Molecular Research Center (1 mentions) Epiquik m6a methylation quantification kit, by Epigentek (1 mentions) Actinomycin d, by GLPBIO (1 mentions)

2 protocols using epigenase m6a methylase activity inhibition assay kit

1

n6-Methyladenosine Methylation Assay

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Sodium arsenite ( NaAsO2 ; #SLBW7984) and FA (#F7876) were obtained from Sigma. SAM (#B9003S) was obtained from New England Biolabs. Actinomycin D (#GC16866) was obtained from GlpBio. Epigenase m6A methylase activity/inhibition assay kit (#P9019-96) and EpiQuik m6A methylation quantification kit (#P-9005-48) were purchased from Epigentek. NE-PER nuclear and cytoplasmic extraction reagents (#78835) were purchased from Thermo Scientific. TRI reagent (#TR-118) was purchased from Molecular Research Center, Inc. The cysteine-rich angiogenic inducer 61 (CYR61) ELISA kit (#CSB-E13884h) was purchased from Cusabio.
Song Y.P., Lv J.W., Zhang Z.C., Qian Q.H., Fan Y.J., Chen D.Z., Zhang H., Xu F.X., Zhang C., Huang Y., Wang H., Wei W, & Xu D.X. (2023). Effects of Gestational Arsenic Exposures on Placental and Fetal Development in Mice: The Role of Cyr61. Environmental Health Perspectives, 131(9), 097004.
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2

Quantifying m6A Methyltransferase Activity

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The Epigenase™ m6A Methylase Activity/Inhibition Assay Kit (#P-9019, EpigenTek, USA) was used to specifically measure the catalytic activity of total m6A methyltransferases. The methylated m6A in the substrate can be recognized by a high affinity m6A antibody. The proportion or amount of methylated m6A is directly proportional to enzyme activity. Readings were taken at 450 nm using a spectrophotometer, and the methyltransferase activity was calculated according to the instructions provided with the kit.
Cheng S., Chen L., Ying J., Wang Y., Jiang W., Zhang Q., Zhang H., Wang J., Wang C., Wu H., Ye J, & Zhang L. (2023). 20(S)-ginsenoside Rh2 ameliorates ATRA resistance in APL by modulating lactylation-driven METTL3. Journal of Ginseng Research, 48(3), 298-309.
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