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Human il 8 elisa max deluxe kit

Manufactured by BioLegend
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The Human IL-8 ELISA MAX™ Deluxe kit is a laboratory assay designed to quantify the levels of human interleukin-8 (IL-8) in biological samples. It is a sandwich enzyme-linked immunosorbent assay (ELISA) that utilizes a pair of highly specific antibodies to capture and detect IL-8 present in the sample.

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Human IL-8 ELISA MAX™ Deluxe Human IL-8 ELISA MAX™ Human IL-8 ELISA kit ELISA MAX Deluxe IL-8 ELISA kit Human IL-8 ELISA MAX kits ELISAs

2 protocols using human il 8 elisa max deluxe kit

1

IL-8 Protein Quantification by ELISA

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IL-8 protein secreted to the conditioned medium was assayed by Human IL-8 ELISA MAX™ Deluxe kit (Biolegend, San Diego, CA) according to manufacturer's instruction. A standard curve of absorbance reading versus the different concentration of recombinant IL-8 standards was plotted. The concentration of IL-8 protein secreted to conditioned medium was determined by comparing the absorbance reading of the sample to the standard curve.
Lau W.H., Pandey V., Kong X., Wang X.N., Wu Z., Zhu T, & Lobie P.E. (2015). Trefoil Factor-3 (TFF3) Stimulates De Novo Angiogenesis in Mammary Carcinoma both Directly and Indirectly via IL-8/CXCR2. PLoS ONE, 10(11), e0141947.
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2

Quantifying Secreted IL-8 Levels

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Secreted IL-8 protein levels were determined using commercially available Human IL-8 ELISA MAX Deluxe kit (BioLegend #431504) as per manufacturer’s instructions. EndoC-βH2 cells were treated with pH 7.4 or 6.4 or with 100 ng/ml PMA (at pH7.4) and culture supernatants were collected and stored for ELISA.
Chandra V., Karamitri A., Richards P., Cormier F., Ramond C., Jockers R., Armanet M., Albagli-Curiel O, & Scharfmann R. (2016). Extracellular acidification stimulates GPR68 mediated IL-8 production in human pancreatic β cells. Scientific Reports, 6, 25765.
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