C57BL/6 and BALB/c mice were primed with rAd5-p24 and CNS-boosted with HIV-VLPs. After 30 days, C57BL/6 animals were injected intracranially with microglia from C57BL/6 and PD-L1 KO animals-labeled with CFSE and pulsed with YI9 peptide (1 μg/ml), while the BALB/c animals were injected with microglial cells from BALB/c mice labeled with CFSE and pulsed with AI9 (1 μg/ml) ± α-PD-L1 (10 μg/ml; M1H5 clone; eBioscience). The CFSE labeling and peptide pulse was carried out as described in the previous section. BMNCs were isolated after 72 hr and the CFSE-hi/CFSE-lo ratio was determined by flow cytometry. Percent CTL activity was calculated using the same formula as described before.
α pd l1
Manufactured by Thermo Fisher Scientific
α-PD-L1 is a recombinant protein that binds to the PD-1 receptor. It is used in research applications to study the PD-1/PD-L1 signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
M1h5 clone, by Thermo Fisher Scientific (1 mentions)
α cd8, by Thermo Fisher Scientific (1 mentions)
α cd45, by BioLegend (1 mentions)
Facsuite software, by BD (1 mentions)
Facsverse flow cytometer, by BD (1 mentions)
α pd 1, by Thermo Fisher Scientific (1 mentions)
α pd l1, by BioLegend (1 mentions)
α cd4, by Thermo Fisher Scientific (1 mentions)
α cd19, by Thermo Fisher Scientific (1 mentions)
2 protocols using α pd l1
1
Assessing CD8+ T Cell Responses
2
Multiparameter Flow Cytometry of Lymphoid Tissues
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Flow cytometric analysis of single-cell suspensions from lymph nodes, spleen, tumor-infiltrating lymphocytes (TILs), and tumor cells was performed using α-PD-1, α-PD-L1, α-CD45 (all BioLegend), α-CD3 (BD Pharmingen), α-CD4, α-CD8, α-CD19 (all eBioscience), α-PD-1-AF680, and α-PD-L1-AF680. Adipocytes were identified after BAT dissociation (as described above) and subsequent staining with α-PAT-2 (mouse IgM; Santa Cruz) and α-mouse IgM (eBioscience) as secondary antibodies. Cells were analyzed using a BD FACSVerse flow cytometer with FACSuite software (Becton Dickinson).
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