Adc enrichment

Difco Middlebrook 7H10 agar, Middlebrook 7H9 broth, Tryptic soy agar, Tryptic soy broth, MOPS, tris(hydroxymethyl)aminomethane, 2-mercaptoethanol, sucrose and triton-X 100 were purchased from Sigma-Aldrich. ADC enrichment was purchased from Fisher Scientific. Magnesium chloride and potassium chloride were obtained from VWR. All reagents and solvents were commercial grade and were used as received without further purification unless otherwise noted. Flash chromatography was performed with Whatman silica gel (Purasil 60 Å, 230-400 Mesh). Analytical thin-layer chromatography was performed with 0.25 mm coated commercial silica gel plates (EMD, Silica Gel 60F₂₅₄) visualizing at 254 nm, or developed with ceric ammonium molybdate or anisaldehyde solutions by heating on a hot plate. ¹H-NMR spectral data were obtained using 400, and 500 MHz instruments. ¹³C-NMR spectral data were obtained using 100 and 125 MHz instruments. For all NMR spectra, δ values are given in ppm and J values in Hz.

Difco Middlebrook 7H10 agar, Middlebrook 7H9 broth, Tryptic soy agar, Tryptic soy broth, MOPS, tris(hydroxymethyl)aminomethane, 2-mercaptoethanol, sucrose and triton-X 100 were purchased from Sigma-Aldrich. ADC enrichment was purchased from Fisher Scientific. Magnesium chloride and potassium chloride were obtained from VWR. All reagents and solvents were commercial grade and were used as received without further purification unless otherwise noted. Flash chromatography was performed with Whatman silica gel (Purasil 60 Å, 230–400 Mesh). Analytical thin-layer chromatography was performed with 0.25 mm coated commercial silica gel plates (EMD, Silica Gel 60F₂₅₄) visualizing at 254 nm, or developed with ceric ammonium molybdate or anisaldehyde solutions by heating on a hot plate. ¹H-NMR spectral data were obtained using 400, and 500 MHz instruments. ¹³C-NMR spectral data were obtained using 100 and125 MHz instruments. For all NMR spectra, δ values are given in ppm and J values in Hz.
Synthesis and chemical characterizations of lipopeptide IIβ (3) and its fluorescent probes, 11 and all new lipopeptide analogs studied in this article are summarized in supporting information.†

Difco Middlebrook 7H10 agar, Middlebrook 7H9 broth, Tryptic soy agar, Tryptic soy broth, MOPS, tris(hydroxymethyl)aminomethane, 2-mercaptoethanol, sucrose and triton-X 100 were purchased from Sigma-Aldrich. ADC enrichment was purchased from Fisher Scientific. Magnesium chloride and potassium chloride were purchased from VWR. All reagents and solvents were commercial grade and were used as received without further purification unless otherwise noted. Flash chromatography was performed with Whatman silica gel (Purasil 60 Å, 230–400 Mesh). Analytical thin-layer chromatography was performed with 0.25 mm coated commercial silica gel plates (EMD, Silica Gel 60F₂₅₄) visualizing at 254 nm, or developed with ceric ammonium molybdate or anisaldehyde solutions by heating on a hot plate. ¹H-NMR spectral data were obtained using 400, and 500 MHz instruments. ¹³C-NMR spectral data were obtained using 100 and125 MHz instruments. For all NMR spectra, δ values are given in ppm and J values in Hz.