Degummed B. mori silkworm silk fiber was supplied by BJ Silk Co. Ltd (Jinju-si, Korea). Calcium chloride dihydrate (71.0%–77.5%) and ethanol (94.5%) were purchased from Samchun Chemical (Pyeongtaek, Korea). Dialysis cellulose membranes were purchased from Sigma-Aldrich Co. (cutoff 12,000–13,000 Da) and Membrane Filtration Products Inc. (Cellu-Sep 5030-43, cutoff 3,500 Da) and presoaked in distilled water for 10 minutes before use. Polyethylene glycol was purchased from KPX Green Chemical Co. Ltd (Seoul, Korea). Phosphate buffer solution (1.0 M, pH 7.4), protease from Streptomyces griseus, rhodamine B (Rho), and fluorescein (Flu) were purchased from Sigma-Aldrich Co. All the chemical agents were used without further purification.
Fluorescein flu
Manufactured by Merck Group
Fluorescein (Flu) is a fluorescent dye used in various laboratory applications. It absorbs light in the blue-green region of the visible spectrum and emits light in the yellow-green region, making it a useful tool for fluorescence-based techniques.
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Lab products found in correlation
2 protocols using fluorescein flu
1
Silk Fiber Regeneration and Labeling
2
Fluorescence Displacement Assays with Co8 Cages
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The water-soluble Co8 cage HW bearing hydroxymethyl substituents that was used for all aqueous solution studies,9b and the analogous unsubstituted cage H that was used for the crystalline sponge experiments,15 (link) were prepared as previously reported. The fluorescent reporters 4-methyl-7-amino-coumarin (MAC ) and fluorescein (FLU ) were obtained from Sigma-Aldrich and used as received. Fluorescence measurements were carried out using either a BMG ClarioStar plate reader with 96-well plates, or an Agilent Cary Eclipse fluorimeter. UV/Vis spectra were obtained using an Implen C40 Nanophotometer. The detailed methodology for the fluorescence displacement assay experiments reported in this paper using either surface-binding FLU ,6 (link) or cavity-binding MAC ,9a is described in detail in Section 2 of the ESI.†
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