Goat anti human fitc

Primary antibodies used in this study included rabbit anti-CD63 (System Biosciences, USA), rabbit anti-CD81 (System Biosciences, USA), rabbit anti-Calnexin (Cell Signaling, China), mouse anti-β-tubulin (Sigma), rabbit anti-phospho-Histone H3 (Ser10) (Milipore Sigma), rabbit anti-Phospho-Stat3 (Tyr705), rabbit anti-STAT3, rabbit anti-Cyclin D1, rabbit anti-Hes1 (Cell Signaling, China). Secondary antibodies used in this study were goat anti-rabbit or mouse HRP, donkey anti-rabbit or mouse Cy5, goat anti-rabbit or mouse Alexa 594, goat anti-human FITC or goat anti-rabbit or mouse Alexa 488 (Jackson Immuno Research, West grove, PA, USA).

Primary antibodies used were rabbit anti-Mad2 (a gift from ED Salmon, Chapel Hill, NC, USA), Rabbit anti-Haspin (a gift from Dr Fangwei Wang, Zhejiang University, China), Mouse anti-BubR1 (BD Transduction, Waltham, MA, USA), Mouse anti-Bub1 (Millipore, Bedford, MA, USA), human anti-centromere (ACA) (Antibodies, Inc., Davis, CA, USA), mouse anti-β-tubulin (Sigma), mouse anti-Aurora A (BD Transduction), rabbit anti-mouse Aurora A (Bethyl, Montgomery, TX, USA), Mouse anti-Aurora B (AIM, BD Transduction), rabbit anti-H3S10ph (Millipore, Bedford, MA, USA), rabbit anti-H3T3ph (Millipore), rabbit anti-H2AT120ph (Active Motif, Carlsbad, CA, USA), mitotic phospho-epitopes (MPM-2; Millipore), rabbit anti-Plk1-pT210 (Epitomics, Cambridge, MA, USA), rabbit anti-AurA-pT288 (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-AurB-pT232 antibody (Abcam, Cambridge, MA, USA), rabbit anti-GFP antibody (Abcam, ab290), rabbit anti-H3 (Abcam, ab1791), mouse anti-GAPDH (TransGen Biotech, Beijing, China), mouse anti-Flag (TransGen Biotech), mouse anti-GST (Proteintech, Wuhan, China). Secondary antibodies used were goat anti-rabbit or mouse HRP, donkey anti-rabbit or mouse Cy5, goat anti-rabbit or mouse Alexa 594, goat anti-human FITC or goat anti-rabbit or mouse Alexa 488 (Jackson Immuno-Research, Westgrove, PA, USA).