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Nlvpmvatv

Manufactured by JPT Peptide Technologies
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Sourced in Germany

NLVPMVATV is a peptide sequence that can be used as a laboratory tool. It functions as a specific epitope for a viral protein.

Automatically generated - may contain errors

Lab products found in correlation

Anti ccr7 bv785, by BioLegend (1 mentions) Anti cd3 bv510 hit3a, by BD (1 mentions) Anti cd8 percp cy5.5 sk1, by BD (1 mentions) Anti cd45ra apc cy7 hi100, by BioLegend (1 mentions)

2 protocols using nlvpmvatv

1

Phenotyping CMV-specific T Cells

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Cryopreserved PBMCs were stained with cell trace violet (CTV; Thermo Fisher) (20 minutes, RT), and then stimulated with 1 μg/mL epitope peptide (NLVPMVATV; JPT Peptide Technologies) for 6 days at 37°C in 500 μL of 10% fetal bovine serum/RPMI 1640 medium (WELGENE). Cells were labeled with HLA-A∗0201-restricted CMVpp65 pentamer (HLA-A2∗0201: CMVpp56495, ProImmune). Next, surface molecule staining, dead cell staining, fixation, permeabilization, and intracellular molecule staining were performed using the following antibodies: anti-CD3-BV510 (HIT3a), anti-CD8-Percp-Cy5.5 (SK1; BD Biosciences), anti-CD19-PE-eFluor610 (HIB19), anti-CD14-PE-eFluor610 (61D3), anti-Ki-67-PE-Cy7 (20Raj1; Thermo Fisher), anti-CD45RA-APC-Cy7 (HI100), and anti-CCR7-BV785 (G043H7; BioLegend), and a Foxp3 Staining Buffer Set. Data were collected using an LSR II instrument.
Kim S., Lee S.W., Koh J.Y., Choi D., Heo M., Chung J.Y., Lee B.H., Yang S.H., Sung Y.C., Lee H., Shin E.C, & Park S.H. (2022). A single administration of hIL-7-hyFc induces long-lasting T-cell expansion with maintained effector functions. Blood Advances, 6(23), 6093-6107.
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2

Purified MART-1 Peptide for MHC Multimers

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HPLC-purified (>80% purity) MART-1 analog peptide (MART-126–35, A27L, ELAGIGILTV) was obtained from LICR (Melbourn-Austin Branch) and ELAGIGILTV-MHC class I multimers were purchased from ProImmune (Oxford, UK). HLA-A2 restricted Influenza (Flu, GILGFVFTL), Epstein-Barr Virus (EBV, GLCTLVAML) and Cytomegalovirus (CMV, NLVPMVATV) peptides were purchased from JPT (JPT Peptide Technologies GmbH, Germany).
Romano E., Michielin O., Voelter V., Laurent J., Bichat H., Stravodimou A., Romero P., Speiser D.E., Triebel F., Leyvraz S, & Harari A. (2014). MART-1 peptide vaccination plus IMP321 (LAG-3Ig fusion protein) in patients receiving autologous PBMCs after lymphodepletion: results of a Phase I trial. Journal of Translational Medicine, 12, 97.
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