Glun1a

Manufactured by Addgene

GluN1a is a subunit of the NMDA receptor, a type of glutamate receptor that is important for synaptic transmission and plasticity in the central nervous system. It plays a critical role in the function of the NMDA receptor, which is involved in various neurological processes.

Automatically generated - may contain errors

Lab products found in correlation

Pfuultra hf, by Agilent Technologies (2 mentions) Glun2a, by Addgene (2 mentions) Glun2b, by Addgene (2 mentions) Pkc γ, by Addgene (2 mentions) Pkc γ dn, by Addgene (2 mentions) T4 dna ligase, by Thermo Fisher Scientific (2 mentions)

2 protocols using glun1a

Constructing TRPM2 and NMDAR Plasmids

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GluN1a (Addgene, 17928), GluN2A (Addgene, 17924), GluN2B (Addgene, 17925), PKC-γ (Addgene, 112266), PKC-γ-DN (Addgene, 21239). The pcDNA4/TO-FLAG-hTRPM2 construct was a kind gift from Dr. Sharenberg AM (University of Washington, Seattle)(Perraud et al., 2003 (link)).
XbaI (BioLabs, R0145S), BamHI (BioLabs, R3136S), XhoI (BioLabs, R0146S), DpnI (BioLabs, R0176S), EcoRI (BioLabs, R3101S), KpnI (BioLabs, R3142S), NotI (BioLabs, R3189S) and T4 DNA ligase (Thermal Fisher Scientific, 2148085), PfuUltra HF (Agilent, 600380–51), and Q5® High-Fidelity DNA Polymerase (Biolabs, M0491S) were used to generating different deletion or mutation constructs.

Zong P., Feng J., Yue Z., Li Y., Wu G., Sun B., He Y., Miller B., Yu A.S., Su Z., Xie J., Mori Y., Hao B, & Yue L. (2022). Functional Coupling of TRPM2 and Extrasynaptic NMDARs Exacerbates Excitotoxicity in Ischemic Brain Injury. Neuron, 110(12), 1944-1958.e8.

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Molecular Manipulation of Ion Channels

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GluN1a (Addgene, 17928), GluN2A (Addgene, 17924), GluN2B (Addgene, 17925), PKC-γ (Addgene, 112266), PKC-γ-DN (Addgene, 21239), CKAR (Addgene, 14860). The pcDNA4/TO-FLAG-hTRPM2 construct was a kind gift from Dr. Sharenberg AM (University of Washington, Seattle).^{51 (link)} DpnI (BioLabs, R0176L) and PfuUltra HF (Agilent, 600380–51) were used to generating different deletion or mutation constructs.

Zong P., Feng J., Legere N., Li Y., Yue Z., Li C.X., Mori Y., Miller B., Hao B, & Yue L. (2024). TRPM2 enhances ischemic excitotoxicity by associating with PKCγ. Cell reports, 43(2), 113722.

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