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Quintix 224 1s

Manufactured by Sartorius
Sourced in Germany

The Quintix 224-1S is a high-precision analytical balance from Sartorius. It has a maximum capacity of 220 grams and a readability of 0.1 milligrams. The balance features a stainless steel weighing platform and a draft shield to ensure accurate measurements.

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4 protocols using quintix 224 1s

1

Leaf Surface Mechanical Force Measurement

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The abaxial side of leaves from 4-week-old plants was physically attached to the measuring pan of the electronic balance QUINTIX224-1S (Sartorius Lab Instruments GmbH & Co., Göttingen, Germany) with surgical tape (3 M Company, MN, USA). The adaxial side of the leaf was treated with 1 falling droplet or brushed once (shown in the above subsections). The peak weight applied to the leaf surface was obtained as the force. The force per unit area (N/m2) is converted from the peak weight (kg) and the contact area of the brush tip (5.6 × 10−5 m2) or raindrop (9.73 × 10−6 m2).
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2

Evaporation Measurement in 3D Printed Microfluidics

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3D printed microfluidic devices (N = 7) with theoretical capacity (~101 ml) facilitated the measurement of the rate of evaporation. Sealing of the inlet and outlet of a device with parafilm after filling with DI water (dyed blue for visualization) formed the complete device for testing. Measurement of the initial sealed device mass (inclusive of water, film, and printed microfluidic system) using a microbalance (Sartorius Quintix 224-1S, Germany) enabled recording of mass loss at 2 and 24 hours. Devices were maintained at room temperature in a controlled laboratory environment reflective of anticipated use environment (22°C, 55% RH). An optical camera (Canon 90D, Canon EF 100 mm f/2.8L USM lens) facilitated observation of visual changes to fluid levels at each measurement interval.
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3

Polymer Consumption Determination Protocol

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The polymer consumption per unit area was determined (a) by measuring the required quantity of casting solution per unit gravimetrically with a Quintix 224-1S laboratory balance (Sartorius AG, Göttingen, Germany) and additionally (b) by the mass difference before and after coating with a XP105 analytical balance (Mettler Toledo, Greifensee, Switzerland). The Software ImageJ 1.51w (by Wayne Rasband) was used to determine the coated surface area by image analysis. All samples were dried under reduced pressure at 60 °C for at least 24 h before further investigations.
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4

Hydration Dynamics of Fern Scales

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Each tissue type was placed in a climate chamber (Binder, KBF 115) and weighted with a balance (Sartorious QUintix224‐1S, 0.1 mg accuracy) across 30–80% rh (at 23 °C) in 10% intervals with 1 h waiting time per interval. The balance logged the weight every minute. The sample of the sclereid cells consisted of two rectangular cuts from the base (where the sclereid layer is the thickest) of two different scales with a combined weight of 92.7 mg. The biggest pieces of the brown tissue of up to three scales were used for this measurement, since it was not possible to prepare one single big piece of brown tissue. One sclerenchyma fiber bundle from one scale was measured (122.0 mg) and again with another approx. half bundle (155.6 mg) from another scale.
In addition, whole scales and dissected tissues adaxial of the sclerenchyma (adaxial epidermis + brown tissue) and abaxial of the sclerenchyma (abaxial epidermis + sclereid), as well as the sclerenchyma itself were submersed in water. The weights of the tissues before and after hydration were measured to obtain the water uptake. The weights after hydration were determined after carefully removing excess water from the respective surfaces using paper towels and waiting an extra minute to let remaining water on the surface evaporate.
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