DMSO, rifampicin, T0901317, SR12813, 17α-ethinylestradiol (EE2), and trans-nonachlor were purchased from Sigma-Aldrich. SPA70, SJB7, and SJC2 were obtained from WuXi AppTec [16 (link)]. All oligonucleotides were from Integrated DNA Technologies and are presented in Supplementary Table 2 .
Spa70
Manufactured by WuXi AppTec
The SPA70 is a laboratory instrument designed for conducting scintillation proximity assays (SPA). It is capable of detecting and measuring radioactive signals generated during these assays, which are commonly used in drug discovery and biochemical research applications. The SPA70 provides a means of quantifying interactions between biomolecules, such as proteins and small molecules, without the need for physical separation.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Rifampicin, by Merck Group (1 mentions)
Sr12813, by Merck Group (1 mentions)
T0901317, by Merck Group (1 mentions)
Trans nonachlor, by Merck Group (1 mentions)
17α ethinylestradiol ee2, by Merck Group (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Opti mem reduced serum medium, by Thermo Fisher Scientific (1 mentions)
Tissue culture flask, by Corning (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Fugene 6, by Promega (1 mentions)
Disposable pipettes, by Corning (1 mentions)
Citco, by Bio-Techne (1 mentions)
Steadylite hts reagent, by PerkinElmer (1 mentions)
2 protocols using spa70
1
Chemical Compound Acquisition for Experimental Research
2
High-Throughput Transfection and Luciferase Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Lipofectamine 3000, penicillin–streptomycin, and puromycin stock solutions; Opti-MEM Reduced Serum Medium; phenol red–free Dulbecco's modified Eagle's medium; and DMSO were all purchased from Thermo Fisher Scientific (Atlanta, GA), and Fugene 6 was purchased from Promega (Madison, WI). Characterized fetal bovine serum (FBS) and charcoal/dextran treated FBS were purchased from HyClone (Logan, UT). Dual-Glo Luciferase Reporter Assay reagent was purchased from Promega (Madison, WI). The 96-well white tissue culture–treated plates and steadylite HTS reagent were purchased from PerkinElmer Life and Analytical Sciences (Boston, MA). Rifampicin (RIF) and CITCO were purchased from Tocris Bioscience (Minneapolis, MN). SPA70 was synthesized by WuXi App Tec (Wuhan, China). All tissue culture supplemental materials, such as tissue culture flasks and disposable pipettes, were purchased from Corning (Corning, NY).
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