Polyoxyethylene 5 nonylphenylether np 5

Zoledronic acid (zoledronate or Zol) monohydrate, alendronic acid (alendronate or Ale) and disodium clodronic acid (clodronate or Clo) tetrahydrate were from TCI America (Portland, OR). Alexa Fluor 647 (AF647)-labeled Zol was from BioVinc LLC (Los Angeles, CA). Sodium hydrate, calcium chloride, zinc chloride, sodium dihydrogen phosphate, sodium hydrogen phosphate, uranyl acetate (UA) dihydrate, formic acid, formalin, collagenase IV, paraformaldehyde, polyoxymethylene, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), polyoxyethylene (5) nonylphenylether (NP-5) and polyoxyethylene (10) octylphenyl ether (OEP-10) were from Sigma-Aldrich (St. Louis, MO). DOPA monosodium salt and 1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG_2K) were from Avanti Polar Lipid, Inc. (Alabaster, AL). Cyclohexane, dimethyl sulfoxide, hexanol, chloroform and ethanol were from Thermo Fisher Scientific Co. (Pittsburgh, PA). APC-labeled anti-mouse CD206 (MMR) antibody was from BioLegend Co. (San Diego, CA). All reagents were used without further purification.

Zoledronic acid monohydrate was from TCI America (Portland, OR). Alexa Fluor 647 (AF647)-labeled Zol (Zol-AF647) was from BioVinc LLC (Culver City, CA). Sodium hydrate, calcium chloride, sodium dihydrogen phosphate, sodium hydrogen phosphate, uranyl acetate (UA) dihydrate, formic acid and polyoxyethylene (5) nonylphenylether (NP-5) were from Sigma-Aldrich (St. Louis, MO). The 1, 2-dioleoyl-sn-glycero-3-phosphate monosodium salt (DOPA) and 1, 2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000 (DSPE-PEG_2K) were from Avanti Polar Lipid, Inc. (Alabaster, AL). Cyclohexane, dimethyl sulfoxide (DMSO), chloroform and ethanol were from Thermo Fisher Scientific Co. (Pittsburgh, PA). All reagents were used without further purification.

Zoledronate monohydrate and Alexa Fluor 647-labeled Zol (Zol-AF647) were
from TCI America (Portland, OR) and BioVinc LLC (Los Angeles, CA), respectively.
Calcium chloride, sodium hydrate, sodium dodecyl sulfate (SDS), sodium hydrogen
phosphate, sodium dihydrogen phosphate, 3-(4, 5-dimethylthiazol-2-yl)-2,
5-diphenyltetrazolium bromide (MTT), formic acid, polyoxyethylene (5)
nonylphenylether (NP-5), and PLGA with molecular weights ranging from 4,000 to
15,000 (average of 9,500) were from Sigma-Aldrich (St. Louis, MO). The
1,2-dioleoyl-sn-glycero-3-phosphate monosodium (DOPA) was
from Avanti Polar Lipid, Inc. (Alabaster, AL). Chloroform, cyclohexane, ethanol
and dimethyl sulfoxide were from Thermo Fisher Scientific Co. (Waltham, MA). Two
types of emulsifying agents, acid-sensitive octadecanoic
acid-hydrazone-PEG_2K and acid-insensitive octadecanoic
acid-amide-PEG_2K, were synthesized and purified as we reported
previously.^{28 (link)–29}