Stains all

Manufactured by Santa Cruz Biotechnology

Stains-All is a laboratory reagent used for staining biomolecules, such as proteins and nucleic acids, in biochemical and molecular biology applications. It functions as a cationic dye that binds to various charged groups on these biological molecules, resulting in a distinctive color change that allows for their visualization and detection.

Automatically generated - may contain errors

Lab products found in correlation

Chef dr 2 system, by Bio-Rad (2 mentions) Seakem gold agarose gel, by Lonza (2 mentions) Chemidoc imaging system, by Bio-Rad (2 mentions)

2 protocols using stains all

Hyaluronan Molecular Weight Assay

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Purified HA was mixed with sucrose solution (final concentration of 333 mM) and loaded to a 0.4% SeaKem Gold agarose gel (Lonza). HA-Ladders (Hyalose) were run alongside the samples. Samples were run 16 h at 9 °C at 4 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run, the gel was stained with 0.005% (w/v) Stains-All (Santa Cruz) in 50% ethanol overnight. Then the gel was washed twice with 10% ethanol for 12h, exposed to light to decrease background, and photographed with ChemiDoc Imaging System (Bio-Rad).

Zhang Z., Tian X., Yuyang Lu J., Boit K., Ablaeva J., Zakusilo F.T., Emmrich S., Firsanov D., Rydkina E., Biashad S.A., Lu Q., Tyshkovskiy A., Gladyshev V.N., Horvath S., Seluanov A, & Gorbunova V. (2023). Increased hyaluronan by naked mole-rat HAS2 improves healthspan in mice. Nature, 621(7977), 196-205.

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Agarose Gel Electrophoresis of Hyaluronic Acid

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Purified HA was mixed with sucrose solution (final concentration of 333 mM) and loaded to a 0.4% SeaKem Gold agarose gel (Lonza). HA-Ladders (Hyalose) were run alongside the samples. Samples were run 12 h at 4 °C at 75 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run, the gel was stained with 0.005% (w/v) Stains-All (Santa Cruz) in 50% ethanol overnight. Then the gel was washed twice with 10% ethanol, exposed to light to decrease background, and photographed with ChemiDoc Imaging System (Bio-Rad).

Takasugi M., Firsanov D., Tombline G., Ning H., Ablaeva J., Seluanov A, & Gorbunova V. (2020). Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties. Nature Communications, 11, 2376.

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