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Anti keratin

Manufactured by Boster Bio
Sourced in China

Anti-keratin is a laboratory reagent used for the detection and quantification of keratin, a structural protein found in various tissues, including skin, hair, and nails. It is commonly used in immunohistochemistry, Western blotting, and other analytical techniques to study the expression and distribution of keratin in biological samples.

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2 protocols using anti keratin

1

Immunofluorescence Staining of HDPFs

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HDPFs at the third passage were fixed with 4 % paraformaldehyde (PFA) for 30 min at room temperature and then incubated in PBS containing 0.4 % Triton X-100 for 10 min on ice and then blocked with 2 % bovine serum albumin (BSA) for 60 min at 37 °C.
After the blocking step, the cells were incubated with primary antibody-anti-vimentin (1:100, Boster, Wuhan, China), anti-keratin (1:100, Boster) at 4 °C overnight; PBS was used as the negative control. The cells were then washed with PBS and incubated for 1 h with the secondary antibodies, namely anti-mouse IgG Alexa Fluor-488 or at antirabbit IgG Alexa Fluor-594 1:1000 at room temperature (Life Technologies, Paisley, UK). Glass cover slips were mounted using mounting media supplemented with DAPI stain (VectorLabs, Peterborough, UK) and preparations imaged under a fluorescent microscope (Olympus, Tokyo, Japan).
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2

Immunofluorescence Assay for Podocyte Characterization

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Podocytes in six-well plates were xed with 4% paraformaldehyde at room temperature for 30 min, incubated in phosphate-buffered saline (PBS) containing 0.4% Triton X-100 for 10 min, and blocked with 2% bovine serum albumin at 37°C for 60 min. The cells were then incubated with the primary antibodies anti-vimentin (1:100; Boster Biological Technology, Wuhan, China) and anti-keratin (1:100; Boster Biological Technology) at 4°C overnight. PBS was used as the negative control. Then, the cells were washed with PBS and incubated with anti-mouse IgG Alexa Fluor 488 or anti-rabbit IgG Alexa Fluor 594 (1:1000; Life Technologies, Paisley, UK) for 1 h at room temperature. The glass cover slides were installed using the installation medium with 4 ,6-diamidino-2-phenylindole dye (Vector Labs, Peterborough, UK) and prepared for imaging under a uorescence microscope under ⊆200 magni cation (Olympus, Tokyo, Japan).
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