Caspase 3 activity assay kit fluorometric

Manufactured by Abcam

The Caspase-3 Activity Assay Kit (Fluorometric) is a laboratory tool designed to measure the activity of caspase-3, a key enzyme involved in the apoptosis (programmed cell death) pathway. The kit utilizes a fluorogenic substrate that emits a fluorescent signal upon cleavage by active caspase-3, allowing for the quantification of caspase-3 activity in cell or tissue samples.

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2 protocols using caspase 3 activity assay kit fluorometric

Caspase 3 Activity Assay Protocol

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Caspase 3 activity was measured
using the Caspase-3 Activity Assay Kit (Fluorometric) (Abcam) as per
the manufacturer’s instructions. Cells were treated with BOS
overnight prior to caspase 3 activity measurement. The FMK caspase
inhibitor (50 mM) was added as a negative control.

da Silva R.A., Stocks C.J., Hu G., Kline K.A, & Chen J. (2024). Bosutinib Stimulates Macrophage Survival, Phagocytosis, and Intracellular Killing of Bacteria. ACS Infectious Diseases, 10(5), 1725-1738.

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Caspase-3 Activity Assay Protocol

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The activity of caspase-3 was measured using the caspase-3 Activity Assay kit (Fluorometric; Abcam) according to the manufacturer's instructions. Experiments were repeated ≥3 times.

Zhuang Q., Jin Z., Zheng X., Jin T, & Xiang L. (2021). Long non-coding RNA LINC00460 serves as a potential biomarker and oncogene via regulation of the miR-320b/PBX3 axis in acute myeloid leukemia. Molecular Medicine Reports, 23(6), 435.

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