The following antibodies were used for immunoblotting: Anti-mouse IgG (H + L; 4410S; 1:5,000), mouse monoclonal α-tubulin antibody (76031; 1:1,000), rabbit monoclonal UHRF1 antibody (87632; 1:1,000), and HA rabbit monoclonal antibody (3724; 1:1,000) were purchased from Cell Signal Transduction (CST). Rabbit polyclonal EG5 antibody (A7907; 1:1,000) and rabbit polyclonal TPX2 antibody (A18327; 1:1,000) were purchased from ABclonal Technology. β-Actin mouse monoclonal antibody (47778; 1:5,000) was purchased from Santa Cruz Biotechnology. Anti-rabbit IgG (H + L; 30000-0-AP; 1:5,000), Flag rabbit polyclonal antibody (20543-1-AP; 1:1,000), and His mouse monoclonal antibody (66005-1-lg; 1:1,000) were purchased from Proteintech.
For immunofluorescence, we employed a mouse monoclonal α-tubulin antibody (76031; 1:100) that was purchased from Cell Signal Transduction (CST). Rabbit polyclonal EG5 antibody (A7907; 1:100) and rabbit polyclonal TPX2 antibody (A18327; 1:100) were purchased from ABclonal Technology. Secondary antibodies labeled with AlexaFluor goat anti-mouse 488 (53-9760-82; 1:500) and goat anti-rabbit 594 (A-21312; 1:500) were purchased from Invitrogen.
For immunofluorescence, we employed a mouse monoclonal α-tubulin antibody (76031; 1:100) that was purchased from Cell Signal Transduction (CST). Rabbit polyclonal EG5 antibody (A7907; 1:100) and rabbit polyclonal TPX2 antibody (A18327; 1:100) were purchased from ABclonal Technology. Secondary antibodies labeled with AlexaFluor goat anti-mouse 488 (53-9760-82; 1:500) and goat anti-rabbit 594 (A-21312; 1:500) were purchased from Invitrogen.