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Complete freund s adjuvant f5881

Manufactured by Merck Group
Sourced in United States

Complete Freund's adjuvant (F5881) is a laboratory reagent commonly used in immunological research to enhance the immune response to an antigen. It is a stable water-in-oil emulsion composed of mineral oil, surfactant, and heat-killed Mycobacterium tuberculosis. The product is designed for research use only and its core function is to stimulate the immune system.

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2 protocols using complete freund s adjuvant f5881

1

Piperine Administration in Neuroinflammation Model

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Piperine (94.62.2, > 98% purity), Complete Freund's adjuvant (F5881) and Pertussis toxin (P7208) were purchased from Sigma-Aldrich (St.Louis, Mo, USA). Antibodies including: anti-myelin basic protein (MBP) (MAB386, Millipore), anti-glial brillary acidic protein (GFAP) (Z0334-Dako), anti-Iba1 (019-19741-Wako), anti-CD45 (14-0451-82 -Invitrogen), anti-Caspase-3 (9661-Cell signaling) and anti-NeuN (MAB-377-Millipore) were used for immunohistochemistry assays. All information related to primary and secondary antibodies are available in Table S1. Piperine dose was selected based on our previous study [30] .
Piperine was freshly prepared by dissolving in Tween 80 and diluting in 0.9% saline ( nal concentration of solvent 0.2%).
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2

Piperine Administration in Neuroinflammation Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
Piperine (94.62.2, > 98% purity), Complete Freund's adjuvant (F5881) and Pertussis toxin (P7208) were purchased from Sigma-Aldrich (St.Louis, Mo, USA). Antibodies including: anti-myelin basic protein (MBP) (MAB386, Millipore), anti-glial brillary acidic protein (GFAP) (Z0334-Dako), anti-Iba1 (019-19741-Wako), anti-CD45 (14-0451-82 -Invitrogen), anti-Caspase-3 (9661-Cell signaling) and anti-NeuN (MAB-377-Millipore) were used for immunohistochemistry assays. All information related to primary and secondary antibodies are available in Table S1. Piperine dose was selected based on our previous study [30] .
Piperine was freshly prepared by dissolving in Tween 80 and diluting in 0.9% saline ( nal concentration of solvent 0.2%).
+ Open protocol
+ Expand

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