Ultrapure urea

Manufactured by Thermo Fisher Scientific

Sourced in United States, Belgium

Ultrapure urea is a high-purity chemical compound used in various laboratory applications. It serves as a primary component in numerous buffer and reagent formulations, contributing to the stability and performance of analytical and experimental procedures.

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Lab products found in correlation

5 protocols using ultrapure urea

Ultrapure Urea Protocol

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Ultrapure urea was purchased from Thermo Fisher Scientific. Unless
otherwise noted, all additional chemicals/reagents and equipment were procured
from Sigma-Aldrich and VWR International, respectively.

Dean D.N, & Lee J.C. (2021). Purification and Characterization of an Amyloidogenic Repeat Domain from the Functional Amyloid Pmel17. Protein expression and purification, 187, 105944.

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Two-Dimensional Gel Electrophoresis Protocols

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Acrylamide/bis solution 40%, ReadyStrip™ IPG (Immobilized pH gradient) strips 17 cm and pH 4-7, TEMED (tetramethylenediamine), ammonium persulfate (APS), 2-mercapto ethanol, iodoacetamide, equilibration buffer I and II, mineral oil, overlay agarose, Precision Plus Protein™ Dual Color Standards, Precision Plus Protein™ Dual Xtra Prestained Protein Standards, Aurum™ Serum Protein Mini Kit are from BioRad (Hercules, CA, USA). Phosphate Buffered Saline (PBS)is from Gibco (Grand Island, NY, USA). Glycine, acetone, and ethanol are from Fisher Scientific (Hampton, NH, USA). Acetonitrile (ACN) and hydrochloric acid (HCl) are from Merck Millipore (Frankfurt, Germany). Glycerol molecular biology grade is from Promega (Madison, WI, USA). SDS (sodium dodecyl sulphate) and trifluoroacetic acid (TFA) are from Sigma–Aldrich (St Louis, MO, USA). Ultrapure Urea, ZOOM Thiourea, Ultrapure Tris, ZOOM CHAPS (3-(3-Cholamidopropyl)dimethylammonio-1-propanesulfonate), dithiothreitol (DTT), and SilverQuest™ Silver Staining Kit are from Thermo Fischer Scientific (Waltham, MA, USA).

Duarte-Delgado N.P., Lujan T.P., Arbeláez-Cortés Á., García-Valencia J., Zapata A., Rojas M., Restrepo-Escobar M., Vásquez G, & Ortiz-Reyes B.L. (2018). Identification of Levels of Serum Amyloid A and Apolipoprotein A1 in Serum Proteomic Analysis of Neuropsychiatric Systemic Lupus Erythematosus Patients. Autoimmune Diseases, 2018, 6728541.

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Quantitative Proteomics Workflow

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Dithiothreitol (DTT), 2-chloroacetamide, Tris-HCl, EDTA, aprotinin, leupeptin, PR-619, PMSF, and LC-MS-grade water were purchased from Sigma-Aldrich (St. Louis, MO). Ultra-pure urea, NaCl, dimethyl pimelimidate, LC-MS grade acetonitrile, trifluoroacetic acid (TFA), formic acid (FA), PepClean C18 spin columns and Empore C18 solid-phase extraction disks were purchased from Thermo Fisher Scientific (Fair Lawn, NJ). Sequencing grade modified trypsin was purchased from Promega (Madison, WI). Sep-Pak SPE C18 cartridges (50 mg, 1 cc) were obtained from Waters (Milford, MA). iTRAQ 4-plex reagent kits were purchased from AB Sciex (Framingham, MA).

Thomas S.N., Zhang H, & Cotter R.J. (2015). Application of quantitative proteomics to the integrated analysis of the ubiquitylated and global proteomes of xenograft tumor tissues. Clinical Proteomics, 12(1), 14.

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Optimizing Microencapsulation of Diverse Compounds

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Triethylene glycol dimethacrylate (TEGDMA), poly (ethylene maleic anhydride) (pEMA; M.W: 100,000 to 500,000) (pEMA), Resorcinol (ACS Reagent ≥ 99%), and 4-(Dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM dye) were procured from Sigma Aldrich (Saint Louis, MO, USA). N, N-Dimethylacrylamide (DMAM; 99% purity) was obtained from Acros Organics (Geel, Antwerpen, Belgium), urea (Ultrapure™ Urea) from Invitrogen (Waltham, MA, USA), and ammonium chloride and formaldehyde (37%) from Fisher Scientific (Hampton, NH, USA). All chemicals were used without further purification.
To achieve the goal of this study, the research plan was divided into three phases. Firstly, the core/shell ratio was optimized. Secondly, the three most common microemulsification techniques were tested using cargo with different water affinities. Finally, in the last phase, the stability of the synthesized microcapsules was assessed by storing them under different conditions. Figure 1 outlines the main parameters studied in this research.

Rajasekaran S.P., Huynh B, & Fugolin A.P. (2024). Tailoring Microemulsification Techniques for the Encapsulation of Diverse Cargo: A Systematic Analysis of Poly (Urea-Formaldehyde) Microcapsules. Journal of Functional Biomaterials, 15(5), 117.

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Factor Xa Chromogenic Substrate Assay

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Chromogenic substrate CH₃SO₂‐(D)‐CHG‐Gly‐Arg‐pNa (Pefachrome FIXa) was obtained from Pentapharm and S‐2765 containing the thrombin inhibitor I‐2581 was from Chromogenix. H‐Glu‐Gly‐Arg‐chloromethylketone (EGRck) was from Bachem. Calcium chloride 1 M solution and deuterium oxide 99.9% were from Sigma‐Aldrich. Ultrapure urea, Molecular Biology grade 5 M NaCl solution, and Tris‐HCl were from Invitrogen. Chicken egg L‐α‐phosphatidylcholine (PC) and porcine brain L‐α‐phosphatidylserine were from Avanti Polar Lipids Inc. N‐2 hydroxyethylpiperazine‐N’‐ethanesulfonic acid (HEPES) was from Serva. Tris(2‐carboxyethyl)phosphine hydrochloride, the TMTduplex Isobaric Tagging kit, Zeba Spin columns (7 K, 0.5 mL), and chymotrypsin were from Thermo Scientific. All other chemicals were from Merck (Darmstadt, Germany).
Images of crystal structures were processed in PyMol v2.4 supplied by Schrӧdinger. Peaks 7.0 software was used for peptide identification was from Bioinformatics Solution Inc. Deuteration level percentages were calculated using HDeXaminer 2.2.0 software (Sierra Analytics).

Freato N., van Alphen F.P., Boon‐Spijker M., van den Biggelaar M., Meijer A.B., Mertens K, & Ebberink E.H. (2021). Probing activation‐driven changes in coagulation factor IX by mass spectrometry. Journal of Thrombosis and Haemostasis, 19(6), 1447-1459.

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