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Accuzol kit

Manufactured by Bioneer
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The AccuZolTM kit is a reagent designed for the isolation and purification of high-quality total RNA from a variety of biological samples, including cells, tissues, and microorganisms. The kit utilizes a proprietary formulation to effectively lyse samples and denature proteins, while preserving the integrity of the extracted RNA.

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Lab products found in correlation

Rnase free dnase, by Thermo Fisher Scientific (2 mentions) Nanodrop np80 spectrophotometer, by Implen (2 mentions)

Close spelling variants of this product

AccuZolTM RNA extraction kit AccuZol Total RNA extraction kit AccuZol™ reagent kit Accuzol

2 protocols using accuzol kit

1

RNA Extraction and cDNA Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The infected leaves and root collars from three biological replicates (one replicate representing a pool of three plants) were flash-frozen after collection and separately ground with a pestle and mortar. Total RNA was extracted using AccuZolTM kit (Bioneer, Korea), according to the manufacturer's instructions. The genomic DNA in the total RNA was eliminated by digestion with RNase-free DNase (Thermo Scientific) following the manufacturer's protocol. The quantity and purity of total RNA was evaluated using a Nanodrop NP80 spectrophotometer (Implen Germany).
The cDNA was synthesized from 5 μg of total RNA using the cDNA Synthesis commercial kit (YTA, Yekta Tajhiz Azma, Iran) according to the manufacturer's instructions.
Hashemi L., Golparvar A.R., Nasr-Esfahani M, & Golabadi M. (2020). Expression analysis of defense-related genes in cucumber (Cucumis sativus L.) against Phytophthora melonis. Molecular biology reports, 47(7).
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2

RNA Extraction and cDNA Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The infected leaves and root collars from three biological replicates (one replicate representing a pool of three plants) were flash-frozen after collection and separately ground with a pestle and mortar. Total RNA was extracted using AccuZolTM kit (Bioneer, Korea), according to the manufacturer's instructions. The genomic DNA in the total RNA was eliminated by digestion with RNase-free DNase (Thermo Scientific) following the manufacturer's protocol. The quantity and purity of total RNA was evaluated using a Nanodrop NP80 spectrophotometer (Implen Germany).
The cDNA was synthesized from 5 μg of total RNA using the cDNA Synthesis commercial kit (YTA, Yekta Tajhiz Azma, Iran) according to the manufacturer's instructions.
Hashemi L., Golparvar A.R., Esfahani M.N., & Golabadi M. (2020). Expression analysis of defense-related genes in cucumber (Cucumis sativus L.) against Phytophthora melonis.
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