We produced incisional hernias in 30 rats according to the model described previously by DuBay et al.10 (link) In an avascular prefascial plane, 6 × 3-cm rectangular full-thickness skin flaps based 2 cm lateral to the ventral midline were raised. This served to separate the skin incisions from the midline laparotomy incision. We then created a 5-cm midline laparotomy incision through the linea alba. This laparotomy incision was repaired with two interrupted, rapidly absorbed, 5-0 plain catgut sutures (Covidien, Mansfield, Mass.) placed approximately 3 mm from the cut fascial edges and equidistant from the incision ends and to each other. The skin flap was then secured in place with three deep 4-0 polyglycolic acid sutures (Polysorb; Covidien), after which the skin incision was closed in running fashion using a 4-0 poly glycolic acid suture. Neck collars were required to prevent autophagy of the incisions. The temporary laparotomy closures successfully produced fascial defects in all 30 animals. By the time the fascial closure failed, herniation of the abdominal viscera was contained by the intact skin flap closure. The incisional hernias were allowed to mature for 28 days before undergoing repair.
Polysorb
Manufactured by Medtronic
Sourced in Ireland, United States, Norway
Polysorb is a lab equipment product manufactured by Medtronic. It is a synthetic, absorbable suture material used for soft tissue approximation and/or ligation, including use in ophthalmic procedures.
Automatically generated - may contain errors
7 protocols using polysorb
1
Incisional Hernia Induction in Rats
2
Measuring Seminiferous Tubule Diameters
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Under optical magnification, seminiferous tubules were divided into 2 groups: the tubules were measured using 5/0 surgical suture (Polysorb, Covidien) which had a diameter of 100 μm. If the diameter difference between the most dilated tubules and the finest tubules was less 50 μm (half of 5/0 surgical suture), we classified as homogeneous tubules (Fig. 2 B). In contrast, if the diameter difference was 50 μm or greater, we classified as heterogeneous tubules (Fig. 2 A). SRR was compared between these 2 groups.
3
Vascularized Hind-Limb Graft Transplantation
4
Experimental Laparotomy in Mice
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Experimental laparotomy was performed as previously described with minor modifications [23 (link)]. Briefly, mice were anesthetized with 2.0% isoflurane (Baxter Healthcare, Puerto Rico, USA) for 2 min, and anesthesia was maintained with 1.4% isoflurane. Mice were placed on a heating pad to maintain body temperature between 36.5–37.0 °C. After the hair was shaved and the surgical field was disinfected, an approximately 1-cm median incision was made 0.5–1 cm below the xiphoid, and approximately 5 cm of the small intestine was gently pulled out and then exposed to sterile gauze presoaked with normal saline. After being rubbed for 10 min, the intestine was returned to the abdomen. The muscle and skin were closed layer-by-layer with 5–0 absorbable sutures (Polysorb, COVIDIEN, USA). Finally, 0.1 ml 0.2% lidocaine was injected subcutaneously for postoperative analgesia. The mice were allowed to recover in an incubator at 35 °C for 30 min before being returned to their home cages.
5
Facial Nerve Injury Model in Mice
6
Surgical Excision of Tumor Xenografts
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Animals with larger tumors (60–100 mm3 on day 8) were treated as previously described and anesthetized to surgically excise tumors on day 14 (day 6 post-treatment). Following a small incision to the skin the tumor was removed using suitable surgical equipment. All blood vessels were closed using a BOVIE Disposable Cautery Pen (Agntho’s AB, Lidingö, Sweden). Last, the surgical incision was closed using Polysorb™, a braided absorbable suture 5–0 from Medtronic (Oslo, Norway) and Histoacryl® biological glue from B. Braun (Melsungen, Germany). Animals that underwent surgery were given Temgesic (from the Hospital Pharmacy at Oslo University Hospital – Radiumhospitalet) subcutaneously (s.c.) twice per day at 8-h intervals (100 μl using a stock solution of 0.3 mg/ml diluted 1:10 in sterile H2O).
7
Applying dCELL for Neourethra Reconstruction
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The dCELL is supplied in small, medium, or large sizes, as either thin (0.2–0.5 mm) or thick (>1.2 mm) variants. For all the cases in this series, we used small (3 × 3 cm) and thin (0.2–0.5 mm) dCELL (Fig. 4 ). Any dCELL that was found to be closer to 0.5 mm thick was easily thinned using Steven’s tenotomy scissors to make it more pliable, thereby allowing it to conform more closely to the contours of the repair. A piece of the dCELL large enough to cover the neourethra (Figs. 5 –7 ) was trimmed from each sheet and sutured into place using a single continuous, peripheral suture of 6/0 or 7/0 Polysorb (Covidien, Medtronic). Once the dCELL was secured, the glans part of the repair was closed over the dCELL and the tourniquet was released to begin the process of achieving hemostasis before final skin closure. At the end of the procedure, the dCELL was palpable as a small raised area underneath the skin closure.
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