The human cell lines used were the hypopharyngeal patient‐derived xenograft cell lines (HPCM1,2 , 11 HPCM 2,2 , 11 HPCM612 cells), which were maintained in RPMI‐1640 medium (Wako) supplemented with 10% fetal bovine serum (FBS), 100 unit/mL penicillin, and 100 μg/mL streptomycin. SK‐MEL‐2‐Luc and MeWo‐Luc cells (human malignant melanoma, maintained in DMEM supplemented with 10% FBS and penicillin/streptomycin) were provided by JCRB. MCC148c cells12 (human lung squamous carcinoma) were maintained in DMEM supplemented with 10% FBS, 0.4 mg/mL hydrocortisone, 2.5 mM Y‐27632 (Focus Biomolecules), and penicillin/streptomycin. DMEM supplemented with 10% FBS and penicillin/streptomycin was used to maintain 293T cells (RIKEN BioResource Center). Chinese hamster ovary (CHO) cells and glycan‐deficient CHO cell lines (Lec1, Lec2, and Lec8) were obtained from the American Type Culture Collection (ATCC) and maintained in RPMI with 10% FBS.
Chinese hamster ovary cells
Manufactured by American Type Culture Collection
Sourced in United States
Chinese hamster ovary (CHO) cells are a commonly used cell line in biotechnology and pharmaceutical research. They are derived from the ovary of the Chinese hamster and are known for their ability to efficiently produce and secrete recombinant proteins. CHO cells are a versatile tool for researchers to study various cellular processes and for the production of therapeutic proteins and other biologics.
Automatically generated - may contain errors
Lab products found in correlation
Y 27632, by Focus Biomolecules (1 mentions)
Rpmi 1640 medium, by Fujifilm (1 mentions)
293t cells, by RIKEN BioResource Center (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Coelenterazine h, by Promega (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Anti ha antibody, by Cell Signaling Technology (1 mentions)
Opti mem 1, by Thermo Fisher Scientific (1 mentions)
Hepes buffered phenol red free medium, by Thermo Fisher Scientific (1 mentions)
Streptavidin phycoerythrin sa pe, by Thermo Fisher Scientific (1 mentions)
Isopropyl beta d thiogalactopyranoside iptg, by Promega (1 mentions)
Anti myc antibody, by Cell Signaling Technology (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Anti ha agarose, by Thermo Fisher Scientific (1 mentions)
Apelin 12, by Phoenix Pharmaceuticals (1 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (1 mentions)
4 protocols using chinese hamster ovary cells
1
Maintenance of Human Cell Lines for Research
2
Murine B7-H4 Immunology Protocol
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BALB/c mice were from the Animal Research Center of Soochow University. The institutional protocol for animal studies was approved by the Institutional Animal Care and Use Committee of the First Affiliated Hospital of Soochow University. Chinese hamster ovary (CHO) cells and mouse myeloma cells (SP2/0 cell line) were from the American Type Culture Collection (ATCC, Manassas, VA, USA). CHO/Mock and CHO/B7-H4 cells were from the investigator's laboratory. All cells were cultured in RPMI-1640 containing 10% fetal bovine sera (FBS).
3
Cell Culture Protocol for RNA-Seq and Transfection
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Immortalized lymphoblastoid cell lines (LCLs; GM06994, GM06991, GM10852, GM07341, GM12250, GM13045, GM06991, GM12045, GM12043, GM10852) from the Utah Residents with European ancestry (CEPH) [70 (link)], were purchased from Coriell Institute for Medical Research. Human cervical cancer cells (HeLa), and Chinese Hamster Ovary cells (CHO) were obtained from American Type Culture Collection. Cells were cultured in DMEM (HeLa), DMEM-F12 (CHO), or RPMI1640 (LCLs), supplemented with 10% fetal bovine serum, 1% penicillin, and 1% streptomycin, in a humidified incubator at 37°C with 5% CO2. For RNA-Seq experiments, cells were grown to 5x105 cells/mL density in T75 tissue culture flask and harvested. For transient transfections, 1.5×106 cells were seeded into 10 cm2 culture plates and after 24 hours transfected with 1 μg plasmid solution containing equal concentration of two expression plasmids carrying either allele of a regulatory variants in a target gene, along with 75 ng emGFP as an internal control, using lipofectamine 2000 reagent (Life Technologies, Foster City, CA).
4
Apelin Receptor Characterization in CHO and HUVEC Cells
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Chinese hamster ovary cells and human umbilical vein endothelial cells were obtained from American Type Culture Collection (ATCC). All restriction enzymes were from NewEngland BioLabs. [Ala13] apelin-13, apelin-12, 13, 15, 17, 36 were purchased from Phoenix Pharmaceuticals. Lipofectamine 2000, streptavidin-phycoerythrin (SA-PE) and Opti-MEM I were obtained from Invitrogen Life Technologies. Isopropyl-beta-D-thiogalactopyranoside (IPTG) and Coelenterazine h were obtained from Promega. HEPES-buffered phenol red−free medium, Dulbecco’s modified eagle medium and Incomplete RPMI-1640 culture medium were purchased from Gibco. Anti-HA-agarose was obtained from Pierce Chemical Co. Polyclonal horseradish peroxidase-conjugated goat anti-rabbit immuno-globulins/HRP was obtained from Zhong Shan Gold Bridge Biology Corporation (China). Anti-Myc antibody and anti-HA antibody were purchased from Cell Signaling Technology
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