U87mg human glioblastoma cell

Manufactured by American Type Culture Collection

Sourced in United States

The U87MG human glioblastoma cells are a widely used in vitro model derived from a human glioblastoma, a type of brain cancer. These cells exhibit characteristics of malignant glioma cells and are commonly used in research related to brain tumors and cancer biology.

Automatically generated - may contain errors

Lab products found in correlation

Dmem medium, by Thermo Fisher Scientific (2 mentions) Matrigel, by BD (2 mentions) Balb c nude mice, by Japan SLC (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Ins 1 rat insulinoma cells, by American Type Culture Collection (1 mentions) Nih 3t3 mouse fibroblast, by American Type Culture Collection (1 mentions) Rat pheochromocytoma pc12 cells, by American Type Culture Collection (1 mentions) Complete rpmi 1640 medium, by Lonza (1 mentions) Mcf 7 breast cancer cells, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Euroclone (1 mentions) L glutamine, by Euroclone (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) U87mg, by BD (1 mentions) Dmem medium, by BD (1 mentions) Hct116 human colorectal carcinoma cells, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

14 protocols using u87mg human glioblastoma cell

Establishment of Xenograft Tumor Models

Check if the same lab product or an alternative is used in the 5 most similar protocols

B16F10 human
melanoma cells,
U87MG human glioblastoma cells, and MDA-231 human breast cancer cells
were purchased from the American Type Culture Collection and grown
in DMEM with 10% fetal bovine serum at 37 °C with 5% CO₂. Cells were used for in vitro or in vivo experiments when they reached
approximately 75% confluence.
Male C57BL/6 Jms and male BALB/c
nude^–/– mice (7-week-old) were purchased
from Japan SLC (Shizuoka, Japan). All animals received humane care,
and the Animal Ethics Committee of the National Institute of Radiological
Sciences approved all experiments. All experiments were carried out
according to the recommendations of the Committee for the Care and
Use of Laboratory Animals, National Institute of Radiological Sciences.
The B16F10 tumor bearing models were established using C57BL/6 J mice
via a left flank subcutaneous injection of B16F10 cells (1 ×
10⁶ cells per mouse) one week before the PET imaging experiment.
The U87MG and MDA-231 tumor bearing models were established using
BALB/c nude^–/– mice via a left hind leg or
left flank subcutaneous injection of U87MG cells (5 × 10⁶ cells per mouse) and MDA-231 cells (5 × 10⁶ cells per mouse), respectively. Tumor bearing mice were used for
studies when tumor diameters reached 3–5 mm.

Hu K., Shang J., Xie L., Hanyu M., Zhang Y., Yang Z., Xu H., Wang L, & Zhang M.R. (2020). PET Imaging of VEGFR with a Novel 64Cu-Labeled Peptide. ACS Omega, 5(15), 8508-8514.

+ Open protocol

+ Expand

Cell Culture Protocols for Glioblastoma, Insulinoma, and HNSCC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The U-87 MG human glioblastoma cells and INS-1 rat insulinoma cells were purchased from the American Type Culture Collection (ATCC, Rockville MD). UM-SCC-22B human head and neck squamous carcinoma cells were purchased from EMD Millipore (Billerica, MA). The cells were grown in Minimum Essential Medium (MEM), RPMI-1640 medium, and Dulbecco's modified Eagle medium (DMEM) respectively, all of which were supplemented with 10% fetal bovine serum, penicillin (100 IU/mL), and streptomycin (100 mg/mL) (Invitrogen, Carlsbad, CA) and maintained in a humidified atmosphere containing 5% CO₂ at 37 °C. Cells were passaged three times per week.

Chen H., Tong X., Lang L., Jacobson O., Yung B.C., Yang X., Bai R., Kiesewetter D.O., Ma Y., Wu H., Niu G, & Chen X. (2017). Quantification of Tumor Vascular Permeability and Blood Volume by Positron Emission Tomography. Theranostics, 7(9), 2363-2376.

+ Open protocol

+ Expand

Glioblastoma Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

U87-MG human glioblastoma cells were originally obtained from American Type Culture Collection (ATCC; Manassas, VA) and cultured as described [15 (link)]. ATCC uses various approaches to verify cell line identity of cell lines and ensure no contaminants are present. Cells were free of mycoplasma upon arrival and were tested periodically thereafter.

Staquicini F.I., Smith T.L., Tang F.H., Gelovani J.G., Giordano R.J., Libutti S.K., Sidman R.L., Cavenee W.K., Arap W, & Pasqualini R. (2019). Targeted AAVP-based therapy in a mouse model of human glioblastoma: a comparison of cytotoxic versus suicide gene delivery strategies. Cancer Gene Therapy, 27(5), 301-310.

+ Open protocol

+ Expand

Glioblastoma Cell Culture Verification

Check if the same lab product or an alternative is used in the 5 most similar protocols

U87-MG human glioblastoma cells were originally obtained from American Type Culture Collection (ATCC; Manassas, VA) and cultured as described (15 (link)). ATCC uses various approaches to verify cell line identity of cell lines and ensure no contaminants are present. Cells were free of mycoplasma upon arrival and were tested periodically thereafter.

+ Open protocol

+ Expand

Cell Culture Conditions for Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

NIH3T3 mouse fibroblast, PC12 rat pheochromocytoma cells, and U87MG human glioblastoma cells were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA). SH-SY5Y cell line was kindly provided by Dr Honglin Zhou at the University of Pennsylvania (Philadelphia, PA, USA). NIH3T3 cells were grown in Dulbecco’s Modified Eagle’s Medium, PC12 cells were grown in RPMI 1640 (Roswell Park Memorial Institute medium 1640), U87MG cells were grown in Modified Eagle’s Medium, and SH-SY5Y cells were grown in a 1:1 mixture of Modified Eagle’s Medium and F12 media. All culture media were supplemented with 10% heat-inactivated fetal bovine serum, 100 μg/mL penicillin, and 100 μg/mL streptomycin. Cultures were maintained at 37°C in a humidified atmosphere of 5% CO₂ and 95% air. For most experiments, unless specified, 5,000/well of logarithmically growing cells were seeded in each of the 384-well plates containing proper medium plus 1% fetal bovine serum with or without compound. Dimethyl sulfoxide ([DMSO] #0.3%) was added to all the control cultures.

Wu J., Wang Y., Liang S, & Ma H. (2014). Cytoprotective effect of selective small-molecule caspase inhibitors against staurosporine-induced apoptosis. Drug Design, Development and Therapy, 8, 583-600.

+ Open protocol

+ Expand

Isolation and Culture of Human Cancer Cells and T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

MCF-7 breast cancer cells and U87 MG human glioblastoma cells were obtained from American Type Culture Collection (ATCC, Rockville, MD). MCF-7 Luciferase cells were kindly provided by Dr. Daniel Vallera (University of Minnesota, Minneapolis, MN). Human cancer lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS, 100 U/mL penicillin, 100 µg/mL streptomycin, and L-glutamine at 37°C in 5% CO₂. Human PBMCs were isolated from buffy coats of healthy donor blood samples (obtained from Memorial Blood Centers, St. Paul, MN) by Ficoll density gradient centrifugation. Unactivated CD8⁺ or CD4⁺ T cells were isolated from PBMCs by positive selection using CD8⁺ or CD4⁺ T cell isolation kit (Invitrogen Life Technologies, Grand Island, NY). PBMCs were cultured in complete RPMI 1640 medium (Lonza) supplemented with 10% (v/v) fetal bovine serum, L-glutamine (final concentration of 2mM), Penicillin (100 units/mL), and Streptomycin (100 µg/mL) in a humidified incubator with 5% CO₂ at 37 ˚C.

Petersburg J., Shen J., Csizmar C.M., Murphy K.A., Spanier J., Gabrielse K., Griffith T.S., Fife B, & Wagner C.R. (2018). Eradication of Established Tumors by Chemically Self-Assembled Nanoring (CSAN) Labelled T Cells. ACS nano, 12(7), 6563-6576.

+ Open protocol

+ Expand

Culture of Human Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

U87MG human glioblastoma cells were purchased from the American Type Culture Collection (ATTC, Manassas, VA, USA). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS, Euroclone, Milan, Italy), 2% penicillin-streptomycin (Sigma-Aldrich), and 2% L-glutamine (Sigma-Aldrich). PANC-1 human pancreatic cancer cell lines (ATCC^® CRL1469™) were cultured in DMEM with sodium pyruvate (Aurogene, Rome, Italy) supplemented with 10% FBS (ATCC), 1% L-glutamine (Euroclone), and 1% antibiotics (Aurogene). Cells were cultivated in T75 flasks and kept at 37 °C, 5% CO₂.

Perini G., Rosa E., Friggeri G., Di Pietro L., Barba M., Parolini O., Ciasca G., Moriconi C., Papi M., De Spirito M, & Palmieri V. (2022). INSIDIA 2.0 High-Throughput Analysis of 3D Cancer Models: Multiparametric Quantification of Graphene Quantum Dots Photothermal Therapy for Glioblastoma and Pancreatic Cancer. International Journal of Molecular Sciences, 23(6), 3217.

+ Open protocol

+ Expand

Establishing U87MG Glioblastoma Xenograft

Check if the same lab product or an alternative is used in the 5 most similar protocols

U87MG human glioblastoma cells were obtained from American Type Culture Collection (Manassas, VA). U87MG tumor model was established by subcutaneous injection of 2×10⁶U87MG tumor cells into the right thighs. All animal experiments were performed in accordance with guidelines of Institutional Animal Care and Use Committee of Peking University. See the supplemental material for the detailed procedures.

Shi J., Fan D., Dong C., Liu H., Jia B., Zhao H., Jin X., Liu Z., Li F, & Wang F. (2014). Anti-tumor Effect of Integrin Targeted 177Lu-3PRGD2 and Combined Therapy with Endostar. Theranostics, 4(3), 256-266.

+ Open protocol

+ Expand

Glioblastoma Tumor Establishment in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

U87MG human glioblastoma cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cultured in DMEM medium (Invitrogen, Carlsbad, CA) with 10% fetal bovine serum at 37 °C in a 5% CO₂ atmosphere. Cells were used for in vitro and in vivo experiments when they reached ~80% confluence. All animal studies were conducted under a protocol approved by the University of Wisconsin Institutional Animal Care and Use Committee. Female athymic nude mice (4-5 weeks old) were purchased from Harlan (Indianapolis, IN) and U87MG tumors were established by subcutaneous (s.c.) injection of 5 × 10⁶ cells, suspended in 100 μL of 1:1 mixture of DMEM medium and Matrigel (BD Biosciences, Franklin lakes, NJ), into the lower right flank of the animal. Tumor size was visually monitored every other day and in vivo experiments were performed when tumors reached 5-10 mm in diameter.

Hernandez R., Valdovinos H.F., Yang Y., Chakravarty R., Hong H., Barnhart T.E, & Cai W. (2014). 44Sc: An Attractive Isotope for Peptide-Based PET Imaging. Molecular Pharmaceutics, 11(8), 2954-2961.

+ Open protocol

+ Expand

Establishing Glioblastoma Xenograft Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

U87MG human glioblastoma
cells were purchased from the American Type Culture Collection (ATCC,
Manassas, VA) and cultured in DMEM medium (Invitrogen, Carlsbad, CA)
with 10% fetal bovine serum at 37 °C in a 5% CO₂ atmosphere.
Cells were used for in vitro and in vivo experiments when they reached ∼80% confluence. All animal
studies were conducted under a protocol approved by the University
of Wisconsin Institutional Animal Care and Use Committee. Female athymic
nude mice (4–5 weeks old) were purchased from Harlan (Indianapolis,
IN), and U87MG tumors were established by subcutaneous (s.c.) injection
of 5 × 10⁶ cells, suspended in 100 μL of 1:1
mixture of DMEM medium and Matrigel (BD Biosciences, Franklin lakes,
NJ), into the lower right flank of the animal. Tumor size was visually
monitored every other day, and in vivo experiments
were performed when tumors reached 5–10 mm in diameter.

Hernandez R., Valdovinos H.F., Yang Y., Chakravarty R., Hong H., Barnhart T.E, & Cai W. (2014). 44Sc: An Attractive Isotope for Peptide-Based PET Imaging. Molecular Pharmaceutics, 11(8), 2954-2961.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!