Tether system

Manufactured by Instech

Sourced in United States

The Tether system is a lab equipment product that provides a secure connection between two or more objects. It is designed to ensure stability and prevent accidental separation during experiments or other lab activities.

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Lab products found in correlation

Citrulline, by Merck Group (1 mentions) Phd 2000 syringe pump, by Harvard Apparatus (1 mentions)

2 protocols using tether system

Intravenous Self-Administration in Rats

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Self-administration procedures were conducted in 12 identical standard rat behavioral test chambers (30.5 × 25.4 × 30.5 cm; Coulbourn Instruments) housed in sound-attenuating cubicles. Each chamber was equipped with two nose-poke holes located on the left and right side of the front wall, which could be illuminated by lights located inside the holes. Twenty milliliter syringes mounted on infusion pumps (Coulbourn Instruments) were used for intravenous drug delivery to rats in each test chamber. The syringes were connected to a tether system (Instech Laboratories) consisting of PE50 tubing that ran from the syringe to a fluid swivel and from there to a fluid line that mated to the back-mounted venous access port. Each chamber was equipped with a liquid dipper trough located in the center of the front wall for delivery of sucrose solution. The chambers were interfaced with a computer running Graphic State 3.0 software to control drug delivery and record nose-poke data from each of the chambers. During self-administration sessions, only one of the two nose-poke holes (the “active” hole) was illuminated (the left/right position of the illuminated hole was counterbalanced across rats and groups and remained constant across all sessions).

Orsini C.A., Colon-Perez L.M., Heshmati S.C., Setlow B, & Febo M. (2018). Functional Connectivity of Chronic Cocaine Use Reveals Progressive Neuroadaptations in Neocortical, Striatal, and Limbic Networks. eNeuro, 5(4), ENEURO.0081-18.2018.

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Sepiapterin and Citrulline Infusion Protocol

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Mice were anesthetized with inhaled isoflurane (4% induction, 2% maintenance) during setup and connection of the infusion line. The catheter was secured in place with a specialized harness (Instech Solomon, Plymouth Meeting, PA, USA) and attached to an infusion line supported by a tether system with a spring-balanced arm and swivel (Instech Solomon, Plymouth Meeting, PA, USA).
Sepiapterin (Schirck’s Laboratories, Jona, Switzerland) and citrulline (Sigma-Aldrich, St. Louis, MO, USA) were dissolved in 0.9% sterile saline (Hospira, Lake Forest, IL, USA), sterilized with a 0.22 μm syringe-tip filter, divided into aliquots, and stored at −80 °C until use. Concentrations of sepiapterin and citruHine and were selected to provide doses of 15 mg kg⁻¹ day⁻¹ and 2.4 g kg⁻¹ day⁻¹ respectively, delivered at a rate of 25 μL/h. Uninfected and infected control mice were infused with 0.9% sterile saline (Hospira, Lake Forest, IL, USA). All infusions were delivered with a PHD2000 syringe pump (Harvard Apparatus, Holliston, MA, USA). Infusions were started the day before inoculation and continued until the mice were sacrificed on day 6 postinoculation.

Alkaitis M.S, & Ackerman H.C. (2016). Tetrahydrobiopterin Supplementation Improves Phenylalanine Metabolism in a Murine Model of Severe Malaria. ACS infectious diseases, 2(11), 827-838.

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