Ht 29 human colon carcinoma cells

HT29 human colon carcinoma cells, H460 human lung carcinoma cells, A549 human lung adenocarcinoma cells, and MIA PaCa-2 human pancreatic carcinoma cells were obtained from the American Type Culture Collection (Manassas, VA, USA). Fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific Inc., Waltham, MA, USA), RPMI-1640 medium (Gibco, Thermo Fisher Scientific Inc.) and Dulbecco's modified Eagle's medium (DMEM; Hyclone; Thermo Fisher Scientific Inc.) were used in the experiments. All antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). APC Annexin V was purchased from Invitrogen (Thermo Fisher Scientific Inc.) and SYTOX Green Nucleic Acid Stain was from BD Biosciences (Franklin Lakes, NJ, USA). Fluorescein isothiocyanate (FITC)-dextran and tCA were purchased from Sigma-Aldrich (St. Louis, MO, USA). EIPA was obtained from Thermo Fisher Scientific Inc. The HDAC-GloTM I/II Assay and Screening system were obtained from Promega Corporation (Waltham, MA, USA).

HT-29 human colon carcinoma cells were obtained from the American Type Culture Collection. For the PrestoBlue assay, HT-29 cells were cultured in 96-well microplates (4,000 cells/well) with DMEM containing 10% fetal bovine serum, 100 U/mL penicillin, and 0.1 mg/mL streptomycin at 37°C in a 5% CO₂/95% air atmosphere. After confluence took place, the cells were exposed to various concentrations of HP-β-CD/CC or SBA-16 (25, 50, 75, 100, 150, and 200 μg/mL prepared in DMEM medium without fetal bovine serum) for 48 hours. Control cells were cultured with DMEM alone. After exposure, the culture medium was replaced with 10% PrestoBlue solution and the cells were incubated for an additional 30 minutes. Finally, the absorbance of each well was measured at 570 nm with a reference wavelength of 600 nm using an enzyme-linked immunosorbent assay reader. The cell viability was calculated as: absorbance of samples ×100/absorbance of control.